CD3-zeta Antibody (F-3): sc-166275

CD3-ζ Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects CD3-ζ protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD3-ζ (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD3-ζ protein plays a crucial role in T cell activation as part of the T cell receptor (TCR) complex, which is essential for the immune response. Located within the plasma membrane of T cells, CD3-ζ protein is integral to the signaling cascade that occurs upon TCR engagement with foreign antigens. This signaling is vital for the activation and proliferation of T cells, enabling them to mount an effective immune response against pathogens. The CD3 complex, which includes CD3-ζ protein, consists of multiple invariant polypeptide chains that form dimers, facilitating the transmission of activation signals through the cell membrane. Notably, CD3-ζ chain contains three immunoreceptor tyrosine-based activation motifs (ITAMs), which are critical for recruiting and activating downstream signaling molecules such as ZAP-70 and Syk, thereby amplifying the T cell activation signal. Understanding the function and importance of CD3-ζ protein is essential for developing therapeutic strategies aimed at modulating immune responses in various diseases, including cancer and autoimmune disorders.

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CD3-zeta Antibody (F-3) References:

1. The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation.  |  Howard, FD., et al. 1992. J Exp Med. 176: 139-45. PMID: 1351920
2. Differential regulation of T-cell receptor processing and surface expression affected by CD3 theta, an alternatively spliced product of the CD3 zeta/eta gene locus.  |  Clayton, LK., et al. 1992. J Biol Chem. 267: 26023-30. PMID: 1464613
3. CD3-zeta surface expression is required for CD4-p56lck-mediated upregulation of T cell antigen receptor-CD3 signaling in T cells.  |  Sancho, J., et al. 1992. J Biol Chem. 267: 7871-9. PMID: 1532798
4. Co-association of CD3 zeta with a receptor (CD16) for IgG Fc on human natural killer cells.  |  Lanier, LL., et al. 1989. Nature. 342: 803-5. PMID: 2532305
5. CD3ζ as a novel predictive biomarker of PD-1 inhibitor resistance in melanoma.  |  Zhang, Z., et al. 2023. Mol Cell Probes. 72: 101925. PMID: 37567322
6. An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination.  |  Zheng, J., et al. 2024. J Immunol Methods. 528: 113664. PMID: 38484791
7. TMX1, a disulfide oxidoreductase, is necessary for T cell function through regulation of CD3ζ.  |  Chai, T., et al. 2024. bioRxiv.. PMID: 39386445
8. CD3 epsilon and CD3 zeta cytoplasmic domains can independently generate signals for T cell development and function.  |  Shinkai, Y., et al. 1995. Immunity. 2: 401-11. PMID: 7719942
9. T cell development in mice lacking the CD3-zeta/eta gene.  |  Malissen, M., et al. 1993. EMBO J. 12: 4347-55. PMID: 8223444
10. CD3 gamma, CD3 delta, and CD3 zeta mRNA in adult human marrow hematopoietic progenitors correlates with surface CD2 and CD7 expression.  |  Long, H., et al. 1996. Exp Hematol. 24: 1402-8. PMID: 8913286

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3-ζ Antibody (F-3) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3-ζ Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3-ζ Antibody (F-3) Specifications

Alternate Product Name: CD247

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 137-163

Epitope Target: CD3-ζ

Epitope Species: human

Product Reactivity/Detection: CD3-ζ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CD3-ζ Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred CD3-ζ antibody is CD3-ζ Antibody (6B10.2): sc-1239.