BPAG1 Double Nickase Plasmid (h): sc-403388-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BPAG1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BPAG1 Double Nickase Plasmid (h) and BPAG1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DST. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BPAG1 Antibody (1B10): sc-293499
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BPAG1 Double Nickase Plasmid (h) | sc-403388-NIC | 20 µg | $410.00 | |||
BPAG1 Double Nickase Plasmid (h2) | sc-403388-NIC-2 | 20 µg | $410.00 |
Dystonin (DST), also known as BPAG1, encodes a large cytoskeletal linker protein of the plakin family that couples intermediate filaments to actin, microtubules, and membrane-associated junctional complexes. In epithelial and neuronal contexts, BPAG1 supports hemidesmosome integrity, cell–matrix adhesion, and cytoskeletal organization, thereby influencing cell polarity, migration, and mechanical stress resilience. DST isoforms participate in coordinated cytoskeletal crosslinking and trafficking processes that affect tissue architecture and axonal stability. Genetic disruption or altered expression of DST has been associated with blistering skin phenotypes and neurodegenerative features, making it a relevant target for studying cytoskeletal failure mechanisms and junctional biology.
BPAG1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DST locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DST. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DST function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DST-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.