Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BChE Double Nickase Plasmid (h): sc-401834-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BChE Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BChE Double Nickase Plasmid (h) and BChE Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BCHE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BChE Antibody (D-5): sc-377403
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BChE Double Nickase Plasmid (h) | sc-401834-NIC | 20 µg | $410.00 | |||
BChE Double Nickase Plasmid (h2) | sc-401834-NIC-2 | 20 µg | $410.00 |
Butyrylcholinesterase (BCHE) encodes BChE, a secreted serine hydrolase abundant in plasma that hydrolyzes choline esters and contributes to systemic cholinergic homeostasis. By scavenging and metabolizing a range of ester substrates, BChE intersects with xenobiotic metabolism and detoxification processes and can influence acetylcholine availability in peripheral tissues. Genetic and expression variation in BCHE has been associated with altered cholinesterase activity and phenotypes relevant to neurobiology, metabolism, and inflammatory responses, making it a useful locus for mechanistic studies. BCHE is also widely used as a functional biomarker gene in studies of cholinesterase regulation and organophosphate sensitivity pathways.
BChE Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BCHE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BCHE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BCHE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BCHE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.