ARMS Double Nickase Plasmid (h): sc-407360-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARMS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARMS Double Nickase Plasmid (h) and ARMS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KIDINS220. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARMS Antibody (26): sc-136462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARMS Double Nickase Plasmid (h) | sc-407360-NIC | 20 µg | $410.00 | |||
ARMS Double Nickase Plasmid (h2) | sc-407360-NIC-2 | 20 µg | $410.00 |
KIDINS220 encodes ARMS (ankyrin repeat-rich membrane spanning protein), a multidomain scaffold that integrates neurotrophin and ephrin receptor signaling to coordinate neuronal differentiation, axon guidance, and synaptic plasticity. ARMS functions downstream of Trk receptors to modulate MAPK/ERK and PI3K–AKT pathways, linking receptor activation to endosomal trafficking and sustained signal propagation. In addition to roles in the nervous system, KIDINS220 contributes to immune cell signaling and cytoskeletal organization through interactions with adaptor proteins and kinases. Genetic disruption or altered expression of KIDINS220 has been associated with neurodevelopmental phenotypes and signaling network dysregulation, making it a useful node for studying receptor-driven pathway wiring.
ARMS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KIDINS220 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KIDINS220. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KIDINS220 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KIDINS220-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.