APPL2 Antibody (E-1): sc-271084

APPL2 Antibody (E-1) is a mouse monoclonal IgG1 antibody that detects APPL2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). APPL2, also known as adaptor protein containing pH domain, PTB domain, and leucine zipper motif 2, plays a crucial role in cellular signaling by linking epidermal growth factor (EGF) signaling to chromatin remodeling within the nucleus. APPL2 is essential for cell growth and proliferation, as APPL2 interacts with the NuRD/MeCP1 complex in the nucleus, facilitating the regulation of gene expression. Additionally, APPL2 associates with the follicle-stimulating hormone receptor (FSHR), highlighting APPL2′s importance in reproductive signaling pathways. Highly expressed in vital organs such as the heart, brain, skeletal muscle, and kidney, APPL2 shares 54% homology with APPL1, underscoring APPL2′s evolutionary significance and functional redundancy. APPL2′s ability to translocate from the plasma membrane to the nucleus, where APPL2 interacts with key regulatory proteins, emphasizes APPL2′s pivotal role in cellular communication and response to external stimuli, making anti-APPL2 antibody (E-1) an invaluable tool for researchers studying these critical biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

APPL2 Antibody (E-1) References:

1. Membrane targeting by APPL1 and APPL2: dynamic scaffolds that oligomerize and bind phosphoinositides.  |  Chial, HJ., et al. 2008. Traffic. 9: 215-29. PMID: 18034774
2. Adaptor protein containing PH domain, PTB domain and leucine zipper (APPL1) regulates the protein level of EGFR by modulating its trafficking.  |  Lee, JR., et al. 2011. Biochem Biophys Res Commun. 415: 206-11. PMID: 22037462
3. Multifunctional protein APPL2 contributes to survival of human glioma cells.  |  Pyrzynska, B., et al. 2013. Mol Oncol. 7: 67-84. PMID: 22989406
4. The tandem PH domain-containing protein 2 (TAPP2) regulates chemokine-induced cytoskeletal reorganization and malignant B cell migration.  |  Li, H., et al. 2013. PLoS One. 8: e57809. PMID: 23460911
5. Association of genetic variation in adaptor protein APPL1/APPL2 loci with non-alcoholic fatty liver disease.  |  Barbieri, M., et al. 2013. PLoS One. 8: e71391. PMID: 23977033
6. The adaptor protein APPL2 inhibits insulin-stimulated glucose uptake by interacting with TBC1D1 in skeletal muscle.  |  Cheng, KK., et al. 2014. Diabetes. 63: 3748-58. PMID: 24879834
7. Rab31 and APPL2 enhance FcγR-mediated phagocytosis through PI3K/Akt signaling in macrophages.  |  Yeo, JC., et al. 2015. Mol Biol Cell. 26: 952-65. PMID: 25568335
8. Adaptor Protein APPL2 Affects Adult Antidepressant Behaviors and Hippocampal Neurogenesis via Regulating the Sensitivity of Glucocorticoid Receptor.  |  Gao, C., et al. 2018. Mol Neurobiol. 55: 5537-5547. PMID: 28965332
9. Ventricular Zone Expressed PH Domain Containing 1 (VEPH1): an adaptor protein capable of modulating multiple signaling transduction pathways during normal and pathological development.  |  Brown, TJ., et al. 2019. Cell Commun Signal. 17: 116. PMID: 31500637
10. The adaptor protein APPL2 controls glucose-stimulated insulin secretion via F-actin remodeling in pancreatic β-cells.  |  Wang, B., et al. 2020. Proc Natl Acad Sci U S A. 117: 28307-28315. PMID: 33122440

The preferred APPL2 antibody is APPL2 Antibody (F-2): sc-398859.