APP/Amyloid Precursor Protein Double Nickase Plasmid (m): sc-419170-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APP/Amyloid Precursor Protein Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APP/Amyloid Precursor Protein Double Nickase Plasmid (m) and APP/Amyloid Precursor Protein Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting App. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β-Amyloid Antibody (B-4): sc-28365
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APP/Amyloid Precursor Protein Double Nickase Plasmid (m) | sc-419170-NIC | 20 µg | $410.00 | |||
APP/Amyloid Precursor Protein Double Nickase Plasmid (m2) | sc-419170-NIC-2 | 20 µg | $410.00 |
Mouse App encodes amyloid precursor protein (APP), a type I transmembrane glycoprotein trafficked through the secretory and endocytic systems where it supports neurite outgrowth, synapse formation, and intracellular signaling. APP is proteolytically processed by α-, β-, and γ-secretases, linking it to regulated intramembrane proteolysis and generation of bioactive fragments that influence neuronal homeostasis. Its processing interfaces with membrane trafficking, lipid raft organization, and calcium-dependent signaling pathways, and is widely studied in the context of neurodegeneration. Dysregulated APP cleavage and altered fragment production are central to mechanistic research on amyloid biology, neuroinflammation, and synaptic dysfunction in Alzheimer’s disease models.
APP/Amyloid Precursor Protein Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the App locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within App. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt App function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of App-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.