APLNR Double Nickase Plasmid (h): sc-402374-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APLNR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APLNR Double Nickase Plasmid (h) and APLNR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APLNR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: APLNR Antibody (3C3-7): sc-517300
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APLNR Double Nickase Plasmid (h) | sc-402374-NIC | 20 µg | $410.00 | |||
APLNR Double Nickase Plasmid (h2) | sc-402374-NIC-2 | 20 µg | $410.00 |
APLNR encodes the apelin receptor, a class A G protein–coupled receptor that binds apelin and Elabela/Toddler peptides to regulate cardiovascular development, angiogenesis, and tissue fluid homeostasis. Upon ligand engagement, APLNR couples predominantly to Gαi and β-arrestin–dependent signaling, modulating pathways such as PI3K–AKT, MAPK/ERK, and nitric oxide signaling that influence endothelial function and cellular migration. Dysregulated APLNR signaling has been implicated in cardiometabolic and vascular biology, including heart failure–associated remodeling, pulmonary hypertension, and tumor-associated angiogenesis, making it a useful node for dissecting microenvironmental signaling. In cell models, perturbation of APLNR supports mechanistic studies of ligand-receptor dynamics, receptor desensitization/internalization, and downstream transcriptional responses.
APLNR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APLNR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APLNR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APLNR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APLNR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.