ADH Antibody (B-12): sc-137078
- ADH Antibody (B-12) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 21-39 near the N-terminus of ADH of human origin
- recommended for detection of ADHα, ADHβ and ADHγ of human origin and Adh1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ADH Antibody (B-12) for WB and IHC(P) applications. These reagents are now offered in bundles with ADH Antibody (B-12) (see ordering information below).
ADH Antibody (B-12) is a mouse monoclonal IgG2a antibody that detects alcohol dehydrogenase (ADH) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ADH antibody (B-12) plays a crucial role in studying alcohol metabolism, as ADH proteins are responsible for metabolizing diverse substrates, including ethanol and other aliphatic alcohols. ADH is primarily located in the liver, where ADH catalyzes the conversion of organic alcohols to ketones or aldehydes in the presence of coenzyme NAD. This liver localization is significant since ADH is essential for removing ethanol produced by intestinal microorganisms. ADH activity is particularly important in preventing toxic metabolite accumulation, which can lead to liver damage and other health issues. The ADH gene cluster, consisting of alpha, beta, and gamma subunits, is organized on chromosome 4q22, with the alpha form being predominant in fetal and infant livers, while beta and gamma forms are more active in adults. Understanding ADH function and regulation is vital for elucidating alcohol metabolism mechanisms and their implications for health and disease.
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ADH Antibody (B-12) References:
- Main ethanol metabolizing alcohol dehydrogenases (ADH I and ADH IV): biochemical functions and the physiological manifestation. | Ashmarin, IP., et al. 2000. FEBS Lett. 486: 49-51. PMID: 11108841
- ADH-1 suppresses N-cadherin-dependent pancreatic cancer progression. | Shintani, Y., et al. 2008. Int J Cancer. 122: 71-7. PMID: 17721921
- Multiple ADH genes are associated with upper aerodigestive cancers. | Hashibe, M., et al. 2008. Nat Genet. 40: 707-9. PMID: 18500343
- Rare ADH variant constellations are specific for alcohol dependence. | Zuo, L., et al. 2013. Alcohol Alcohol. 48: 9-14. PMID: 23019235
- Association between common alcohol dehydrogenase gene (ADH) variants and schizophrenia and autism. | Zuo, L., et al. 2013. Hum Genet. 132: 735-43. PMID: 23468174
- Contrary to consensus, oxidation of ethanol by human alcohol dehydrogenase (ADH) 1A is activated by ATP. | Khmelinskii, I. and Makarov, VI. 2022. Biochimie. 195: 67-70. PMID: 34742856
- Para-endocrine tumor activity with emphasis on ectopic ADH secretion. Genetic, diagnostic, prognostic and therapeutic aspects. | Vorherr, H. 1974. Oncology. 29: 382-416. PMID: 4604870
- Role of cardiovascular receptors in the control of ADH release. | Share, L. 1976. Cardiology. 61 suppl 1: 51-64. PMID: 788911
- Vinblastine and inappropriate ADH secretion. | Ginsberg, SJ., et al. 1977. N Engl J Med. 296: 941. PMID: 846518
- ADH and oxytocin in age-related minor cognitive impairment. | Legros, JJ. 1997. Aging (Milano). 9: 36-7. PMID: 9358876
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADH Antibody (B-12) | sc-137078 | 200 µg/ml | $322.00 | |||
ADH Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-537398 | 200 µg Ab; 10 µg BP | $361.00 | |||
ADH Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-534652 | 200 µg Ab; 40 µg BP | $361.00 | |||
ADH Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-548005 | 200 µg Ab; 10 µg BP | $361.00 | |||
ADH (B-12) Neutralizing Peptide | sc-137078 P | 100 µg/0.5 ml | $69.00 |