Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h): sc-402115-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h) and Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCY1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Adenylate cyclase 1/AC1/ADCY1 Antibody (F-10): sc-365350
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h) | sc-402115-NIC | 20 µg | $410.00 | |||
Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h2) | sc-402115-NIC-2 | 20 µg | $410.00 |
Human ADCY1 encodes adenylate cyclase 1 (AC1), a calmodulin-stimulated membrane enzyme that converts ATP to cAMP in response to calcium signals. AC1-derived cAMP activates PKA and EPAC and modulates downstream CREB-dependent transcription, shaping neuronal excitability, synaptic plasticity, and activity-dependent gene expression. Through integration of Ca2+-coupled GPCR signaling with second-messenger dynamics, ADCY1 influences learning- and memory-associated pathways and broader neurodevelopmental programs. Dysregulated cAMP signaling linked to altered ADCY1 activity has been studied in the context of neurological and neuropsychiatric phenotypes, supporting its relevance for mechanistic investigations.
Adenylate cyclase 1/AC1/ADCY1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCY1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCY1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCY1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCY1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.