TRIM64C Inhibitors
Chemical inhibitors of TRIM64C function to impede the protein's role in various protein degradation pathways by targeting the mechanisms through which it operates. MG132, Lactacystin, Bortezomib, and Epoxomicin are all inhibitors that directly affect the proteasome, a complex responsible for the degradation of proteins that have been tagged for destruction, often by ubiquitination, a process in which TRIM64C is involved. MG132 is a peptide aldehyde that reversibly inhibits the proteasome, while Lactacystin irreversibly binds to the catalytic subunit of the proteasome, providing a more permanent inhibition. Bortezomib, a dipeptide boronic acid, specifically targets the 26S proteasome and Epoxomicin selectively inhibits the chymotrypsin-like activity of the proteasome. Each of these inhibitors leads to the accumulation of proteins that TRIM64C has marked for degradation, effectively impeding TRIM64C's function in the proteasomal degradation pathway.
In addition to proteasomal inhibitors, other chemicals target different aspects of protein degradation pathways involving TRIM64C. Chloroquine and Bafilomycin A1 both disrupt lysosomal degradation, with Chloroquine increasing the pH inside lysosomes and Bafilomycin A1 inhibiting the vacuolar-type H+-ATPase (V-ATPase) responsible for lysosomal acidification. Similarly, Concanamycin A also inhibits V-ATPase. E64 and Leupeptin serve as inhibitors of cysteine and serine proteases, respectively, with E64 being an irreversible inhibitor, which could block the cleavage of proteins post-ubiquitination by TRIM64C. 3-Methyladenine inhibits autophagy by preventing autophagosome formation, a process that can be utilized for the degradation of TRIM64C-tagged proteins. ALLN, or calpain inhibitor I, impedes the function of calpain proteases, which may be involved in the degradation process of TRIM64C's substrates. Lastly, Z-VAD-FMK is a broad-spectrum caspase inhibitor that can hinder the function of caspases, enzymes that may participate in the degradation of proteins during apoptosis.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG132 is a peptide aldehyde that inhibits the proteasomal degradation pathway. Since TRIM64C tags proteins for degradation via the ubiquitin-proteasome system, MG132 would inhibit the degradation of proteins tagged by TRIM64C, leading to the functional inhibition of TRIM64C's role in protein turnover. | ||||||
Lactacystin | 133343-34-7 | sc-3575 sc-3575A | 200 µg 1 mg | $165.00 $575.00 | 60 | |
Lactacystin is a specific inhibitor of the proteasome that irreversibly binds to its catalytic subunit. By inhibiting the proteasome, Lactacystin prevents the degradation of proteins ubiquitinated by TRIM64C, thus inhibiting TRIM64C's function in protein degradation. | ||||||
Bortezomib | 179324-69-7 | sc-217785 sc-217785A | 2.5 mg 25 mg | $132.00 $1064.00 | 115 | |
Bortezomib is a dipeptide boronic acid that inhibits the 26S proteasome. By inhibiting the proteasome, Bortezomib leads to the accumulation of TRIM64C-ubiquitinated proteins, thus inhibiting TRIM64C's role in proteasomal degradation. | ||||||
Epoxomicin | 134381-21-8 | sc-201298C sc-201298 sc-201298A sc-201298B | 50 µg 100 µg 250 µg 500 µg | $134.00 $215.00 $440.00 $496.00 | 19 | |
Epoxomicin is a natural product that selectively inhibits the chymotrypsin-like activity of the proteasome. Inhibition of the proteasome by Epoxomicin would result in the functional inhibition of TRIM64C by preventing the degradation of its ubiquitinated substrates. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $68.00 | 2 | |
Chloroquine functions by increasing the pH inside lysosomes, which can inhibit lysosomal protein degradation pathways. Since TRIM64C may also direct proteins for lysosomal degradation, Chloroquine's action would inhibit this function of TRIM64C. | ||||||
Bafilomycin A1 | 88899-55-2 | sc-201550 sc-201550A sc-201550B sc-201550C | 100 µg 1 mg 5 mg 10 mg | $96.00 $250.00 $750.00 $1428.00 | 280 | |
Bafilomycin A1 is an inhibitor of the vacuolar-type H+-ATPase (V-ATPase). By inhibiting V-ATPase, Bafilomycin A1 prevents acidification of the lysosome, inhibiting lysosomal degradation of proteins tagged by TRIM64C. | ||||||
Concanamycin A | 80890-47-7 | sc-202111 sc-202111A sc-202111B sc-202111C | 50 µg 200 µg 1 mg 5 mg | $65.00 $162.00 $650.00 $2550.00 | 109 | |
Concanamycin A, similar to Bafilomycin A1, inhibits V-ATPase and thus lysosomal acidification. This inhibition would result in the functional inhibition of TRIM64C by blocking the lysosomal degradation pathway it utilizes. | ||||||
E-64 | 66701-25-5 | sc-201276 sc-201276A sc-201276B | 5 mg 25 mg 250 mg | $275.00 $928.00 $1543.00 | 14 | |
E64 is an irreversible inhibitor of cysteine proteases. By inhibiting these proteases, E64 would inhibit the breakdown of proteins that require cysteine protease activity post-ubiquitination by TRIM64C. | ||||||
Leupeptin hemisulfate | 103476-89-7 | sc-295358 sc-295358A sc-295358D sc-295358E sc-295358B sc-295358C | 5 mg 25 mg 50 mg 100 mg 500 mg 10 mg | $72.00 $145.00 $265.00 $489.00 $1399.00 $99.00 | 19 | |
Leupeptin inhibits serine and cysteine proteases. Inhibition of these proteases would prevent the proteolytic processing of TRIM64C's substrates, thus inhibiting TRIM64C's function in protein breakdown. | ||||||
Autophagy Inhibitor, 3-MA | 5142-23-4 | sc-205596 sc-205596A | 50 mg 500 mg | $56.00 $256.00 | 113 | |
3-Methyladenine is an inhibitor of autophagy by blocking autophagosome formation. Since autophagy can be a route of degradation for TRIM64C-tagged proteins, inhibition by 3-Methyladenine would functionally inhibit TRIM64C. | ||||||