SPACA1 Inhibitors
Chemical inhibitors of SPACA1 include a range of compounds that interfere with the protein's function by targeting the actin cytoskeleton, which is crucial for sperm acrosome formation and function. Phalloidin binds to F-actin, stabilizing the filaments and thereby inhibiting SPACA1 by preventing the actin remodeling that is necessary for the acrosome reaction. Similarly, Cytochalasin D impedes actin polymerization, which is essential for maintaining the structure and integrity of the acrosome, ultimately inhibiting SPACA1's role in this process. Latrunculin A also affects actin dynamics by binding to monomeric G-actin, preventing its incorporation into filaments, thus disrupting the actin cytoskeleton organization and SPACA1's associated functions. Jasplakinolide, another stabilizer of actin filaments, prevents the necessary cytoskeletal changes required for SPACA1's involvement in the acrosome reaction, inhibiting its function.
Further inhibition of SPACA1 is achieved through the modulation of the actomyosin system. Blebbistatin, a myosin II ATPase inhibitor, disrupts myosin function, which could be crucial for SPACA1's role in the acrosome reaction. Y-27632, as a ROCK inhibitor, and ML-7, which targets myosin light chain kinase, both inhibit the phosphorylation events that lead to actomyosin contraction, an important aspect of acrosome reaction where SPACA1 might have a role. Wiskostatin and CK-636, through their inhibition of N-WASP and the Arp2/3 complex, respectively, interfere with actin nucleation and branching, processes that are likely essential for SPACA1's function in sperm acrosome integrity. SMIFH2 targets formin-mediated actin assembly, further inhibiting SPACA1 by disrupting the actin structures critical for its activity. Lastly, the modulation of protein phosphorylation states by PKC inhibitors like Chelerythrine and Gö 6983 can inhibit SPACA1. These inhibitors alter the phosphorylation and activity of proteins involved in the regulation of SPACA1, thus impeding its function in the sperm acrosome reaction and fertilization process.
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Items 1 to 10 of 11 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Phalloidin | 17466-45-4 | sc-202763 | 1 mg | $229.00 | 33 | |
Phalloidin binds and stabilizes F-actin, which can inhibit SPACA1 by stabilizing actin filaments and possibly preventing the actin remodeling required for SPACA1's function in sperm acrosome reaction and fertilization. | ||||||
Cytochalasin D | 22144-77-0 | sc-201442 sc-201442A | 1 mg 5 mg | $145.00 $442.00 | 64 | |
Cytochalasin D disrupts actin polymerization. Inhibition of actin filament formation can inhibit SPACA1 by disrupting the actin cytoskeleton, which may be necessary for the proper localization or function of SPACA1 in sperm acrosome formation. | ||||||
Latrunculin A, Latrunculia magnifica | 76343-93-6 | sc-202691 sc-202691B | 100 µg 500 µg | $260.00 $799.00 | 36 | |
Latrunculin A binds to actin monomers and prevents their polymerization. Disruption of actin dynamics can inhibit SPACA1 by interfering with the actin-based processes essential for SPACA1’s role in sperm acrosome integrity and function. | ||||||
Jasplakinolide | 102396-24-7 | sc-202191 sc-202191A | 50 µg 100 µg | $180.00 $299.00 | 59 | |
Jasplakinolide stabilizes actin filaments and can inhibit SPACA1 by preventing the actin cytoskeletal changes necessary for SPACA1's function in the sperm acrosome, potentially impacting acrosome reaction and fertilization processes. | ||||||
(S)-(−)-Blebbistatin | 856925-71-8 | sc-204253 sc-204253A sc-204253B sc-204253C | 1 mg 5 mg 10 mg 25 mg | $71.00 $260.00 $485.00 $949.00 | ||
Blebbistatin inhibits myosin II ATPase activity. By disrupting myosin II function, it can inhibit SPACA1 by potentially affecting the actomyosin interactions and dynamics that SPACA1 may rely on during acrosome reaction in sperm cells. | ||||||
Y-27632, free base | 146986-50-7 | sc-3536 sc-3536A | 5 mg 50 mg | $182.00 $693.00 | 88 | |
Y-27632 is a ROCK inhibitor which can inhibit SPACA1 by impeding the Rho-associated kinase pathway, possibly impacting actin cytoskeleton organization and thus affecting SPACA1’s role within the acrosome of sperm cells. | ||||||
ML-7 hydrochloride | 110448-33-4 | sc-200557 sc-200557A | 10 mg 50 mg | $89.00 $262.00 | 13 | |
ML-7 is an inhibitor of myosin light chain kinase (MLCK). Inhibiting MLCK can inhibit SPACA1 by reducing actomyosin contraction, which could be required for SPACA1's function in acrosome reaction and sperm motility. | ||||||
Wiskostatin | 253449-04-6 | sc-204399 sc-204399A sc-204399B sc-204399C | 1 mg 5 mg 25 mg 50 mg | $48.00 $122.00 $432.00 $812.00 | 4 | |
Wiskostatin inhibits the activity of N-WASP, a regulator of actin polymerization. Inhibiting N-WASP can inhibit SPACA1 by altering actin dynamics, potentially impacting SPACA1's involvement in acrosome formation and function in sperm cells. | ||||||
SMIFH2 | 340316-62-3 | sc-507273 | 5 mg | $140.00 | ||
SMIFH2 is an inhibitor of the formin homology 2 (FH2) domain proteins that nucleate actin filaments. By disrupting formin-mediated actin assembly, it can inhibit SPACA1 by affecting actin structure and dynamics required for SPACA1 function in sperm cells. | ||||||
Chelerythrine chloride | 3895-92-9 | sc-3547 sc-3547A | 5 mg 25 mg | $88.00 $311.00 | 17 | |
Chelerythrine is a potent inhibitor of protein kinase C (PKC). Inhibition of PKC can inhibit SPACA1 by altering the phosphorylation states of proteins involved in the regulation of SPACA1's role in acrosome reaction and sperm function. | ||||||