p100 Activators
p100, also known as NF-κB2 precursor, is a critical component of the nuclear factor-kappa B (NF-κB) signaling pathway, a key regulator of immune responses, inflammation, and cell survival. Functionally, p100 serves as a precursor protein that undergoes proteolytic processing to generate the active form of NF-κB2, p52. The NF-κB pathway is tightly regulated and responds to various extracellular stimuli such as proinflammatory cytokines, microbial pathogens, and cellular stressors. Upon activation, p100 is phosphorylated by the IκB kinase (IKK) complex, which leads to its proteasomal processing by the 26S proteasome. This processing event results in the generation of the p52 subunit, which subsequently translocates to the nucleus and regulates the transcription of target genes involved in immune and inflammatory responses, cell proliferation, and apoptosis.
The activation of p100 and subsequent generation of p52 are tightly regulated processes orchestrated by multiple signaling pathways. In canonical NF-κB signaling, activation typically occurs through the phosphorylation and subsequent degradation of the inhibitor of κB (IκB) proteins, which liberates NF-κB dimers, allowing their translocation to the nucleus and activation of target gene transcription. However, the activation of p100 involves a distinct mechanism, primarily mediated by the noncanonical NF-κB signaling pathway. Upon stimulation by specific ligands such as lymphotoxin beta (LTβ) and B-cell activating factor (BAFF), the noncanonical NF-κB pathway is activated, leading to the activation of NF-κB-inducing kinase (NIK) and subsequent phosphorylation of IKKα. Activated IKKα then phosphorylates p100, targeting it for proteasomal processing and generating the active p52 subunit. Overall, elucidating the precise mechanisms underlying p100 activation provides valuable insights into the regulation of NF-κB signaling and its implications in immune responses and inflammatory diseases.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Leptomycin B | 87081-35-4 | sc-358688 sc-358688A sc-358688B | 50 µg 500 µg 2.5 mg | $107.00 $416.00 $1248.00 | 35 | |
Leptomycin B is a potent inhibitor of CRM1/exportin-1, disrupting nuclear export. p100 undergoes nuclear export through CRM1. Inhibiting CRM1 with Leptomycin B leads to the nuclear accumulation of p100, potentially influencing its activation. Leptomycin B serves as a tool to investigate the regulatory role of nuclear export in p100 function. | ||||||
[4-[(4-Benzo[b]thien-2-yl-2-pyrimidinyl)amino]phenyl][4-(1-pyrrolidinyl)-1-piperidinyl]-methanone | 873225-46-8 | sc-503387 | 10 mg | $430.00 | ||
IKK Inhibitor VII is a selective inhibitor of IκB kinase (IKK), a key regulator of the NF-κB pathway. p100 is processed by IKK to generate p52. Inhibiting IKK with IKK Inhibitor VII potentially influences the processing of p100, modulating NF-κB activation. This compound provides a means to study the impact of IKK inhibition on p100 processing and NF-κB signaling. | ||||||
ML 120B Dihydrochloride | 783348-36-7 (free base) | sc-487759 | 5 mg | $380.00 | ||
MLN120B is a selective inhibitor of IKKβ, a key component of the NF-κB pathway. p100 processing is regulated by IKKβ. Inhibiting IKKβ with MLN120B may impact the processing of p100, influencing NF-κB activation. MLN120B provides a tool to study the specific role of IKKβ in p100 regulation and the downstream effects on NF-κB signaling. | ||||||
Withaferin A | 5119-48-2 | sc-200381 sc-200381A sc-200381B sc-200381C | 1 mg 10 mg 100 mg 1 g | $130.00 $583.00 $4172.00 $20506.00 | 20 | |
Withaferin A is a natural compound with anti-inflammatory properties. It inhibits NF-κB activation by preventing IκBα phosphorylation. p100 processing is regulated by NF-κB. By inhibiting NF-κB with Withaferin A, the processing of p100 may be altered, potentially influencing NF-κB activation. This natural compound offers a tool to explore the anti-inflammatory effects of Withaferin A through p100 regulation. | ||||||
BAY 11-7085 | 196309-76-9 | sc-202490 sc-202490A | 10 mg 50 mg | $124.00 $526.00 | 55 | |
Bay 11-7085 is an inhibitor of IκBα phosphorylation, preventing its degradation. p100 is processed by IKK, regulated by the NF-κB pathway. By inhibiting IκBα phosphorylation, Bay 11-7085 potentially influences p100 processing and NF-κB activation. This compound serves as a tool to study the impact of inhibiting IκBα phosphorylation on p100 regulation and NF-κB signaling. | ||||||
CDDO Methyl Ester | 218600-53-4 | sc-504720 | 10 mg | $220.00 | ||
CDDO-Me is a synthetic triterpenoid with anti-inflammatory properties. It inhibits NF-κB activation by preventing IκBα phosphorylation. p100 processing is regulated by NF-κB. By inhibiting NF-κB with CDDO-Me, the processing of p100 may be altered, potentially influencing NF-κB activation. This compound offers a tool to explore the anti-inflammatory effects of CDDO-Me through p100 regulation. | ||||||