Date published: 2025-12-4

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Hoechsts

Santa Cruz Biotechnology now offers a broad range of Hoechsts for use in various applications. Hoechsts are fluorescent dyes commonly utilized in cell biology and molecular biology research due to their high affinity for DNA. These dyes are particularly valuable in microscopy, flow cytometry, and fluorescence imaging, where they help in visualizing and quantifying nucleic acids within cells. The unique properties of Hoechsts allow them to selectively bind to the minor groove of double-stranded DNA, emitting a bright fluorescence upon binding, which makes them an essential tool for studying cell cycle, apoptosis, and DNA damage. Researchers leverage Hoechsts to track and analyze cell populations, sort cells based on DNA content, and investigate the effects of various treatments on cellular DNA. The specificity and sensitivity of Hoechsts in detecting DNA have made them indispensable in experiments requiring precise nuclear staining and quantification. Santa Cruz Biotechnology's offering of Hoechsts supports a wide array of experimental protocols, catering to the needs of laboratories focused on genetic research, cellular dynamics, and biochemical assays. View detailed information on our available Hoechsts by clicking on the product name.
Product NameCAS #Catalog #QUANTITYPriceCitationsRATING

Hoechst 33342, UltraPure grade

23491-52-3sc-391054
sc-391054A
100 mg
1 g
$145.00
$503.00
8
(2)

Hoechst 33342, UltraPure grade, is a highly selective DNA-binding dye known for its strong affinity for A-T rich regions in double-stranded DNA. This compound exhibits remarkable fluorescence enhancement upon intercalation, leading to distinct emission characteristics. Its unique ability to penetrate live cells allows for real-time visualization of nuclear structures. Additionally, Hoechst 33342 demonstrates low cytotoxicity, making it suitable for various experimental conditions while maintaining robust signal clarity.

Hoechst 33342 trihydrochloride

23491-52-3sc-200908
100 mg
$139.00
15
(1)

Hoechst 33342 trihydrochloride is a fluorescent dye that exhibits a strong preference for binding to adenine-thymine (A-T) rich sequences in DNA, facilitating its use in nucleic acid studies. Its intercalative nature enhances fluorescence intensity, enabling sensitive detection of cellular components. The compound's unique structural properties allow it to traverse cellular membranes, providing insights into nuclear morphology and dynamics. Its stability under various conditions further supports its application in diverse biological assays.

Hoechst 33258, UltraPure grade

23491-45-4sc-391053
sc-391053A
100 mg
1 g
$120.00
$675.00
1
(0)

Hoechst 33258, UltraPure grade, is a fluorescent dye renowned for its selective affinity for double-stranded DNA, particularly in regions rich in adenine and thymine. This compound intercalates between base pairs, resulting in enhanced fluorescence that is sensitive to conformational changes in nucleic acids. Its unique ability to penetrate cellular membranes allows for effective visualization of chromatin structure and dynamics, making it a powerful tool in molecular biology research.

Hoechst 33258

23491-45-4sc-394039
5 ml
$155.00
42
(1)

Hoechst 33258 is a bisbenzimidazole derivative that exhibits strong binding to DNA through intercalation, primarily targeting regions with high adenine-thymine content. This compound's fluorescence is significantly influenced by the local environment, allowing for real-time monitoring of DNA conformational changes. Its distinct ability to form stable complexes with nucleic acids enhances its utility in studies of DNA topology and structural dynamics, providing insights into molecular interactions at the cellular level.