FAM159B Activators
Chemical activators of shisa like 2B can facilitate its functional activation through various biochemical pathways. Forskolin, by elevating cAMP levels, can activate protein kinase A, which is known to phosphorylate target proteins, thereby enhancing their activity and protein-protein interactions. Similarly, the activation of protein kinase C by Phorbol 12-myristate 13-acetate (PMA) leads to the phosphorylation of shisa like 2B. This phosphorylation alters the protein's conformation and its interaction with other cellular components, ultimately increasing its activity within the cell. Ionomycin, by raising intracellular calcium levels, prompts the activation of calmodulin-dependent kinases, which can also result in the phosphorylation and subsequent activation of shisa like 2B.
Inhibition of protein phosphatases plays a pivotal role in the regulation of shisa like 2B activity. Both Okadaic acid and Calyculin A inhibit protein phosphatases such as PP1 and PP2A, which usually dephosphorylate cellular proteins. Their inhibition leads to sustained phosphorylation, which can maintain shisa like 2B in an active state. Epigallocatechin gallate (EGCG) activates AMP-activated protein kinase (AMPK), which may phosphorylate and activate shisa like 2B. Anisomycin, through its activation of JNK pathway, can lead to the phosphorylation and activation of shisa like 2B. Spermidine induces autophagy, which might result in the degradation of inhibitors of shisa like 2B, thereby increasing its activity. Resveratrol activates SIRT1, which can deacetylate proteins and may thus enhance the function of shisa like 2B. Lithium chloride, by inhibiting GSK-3β, can lead to the activation of proteins through the inactivation of their inhibitors, indirectly affecting the activity state of shisa like 2B. Lastly, sodium butyrate, as a histone deacetylase inhibitor, can lead to changes in gene expression that enhance the activation of shisa like 2B, while 5-Azacytidine, by inhibiting DNA methyltransferases, can activate genes that encode for proteins that interact with and activate shisa like 2B.
SEE ALSO...
Items 1 to 10 of 11 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which can then phosphorylate shisa like 2B, leading to its functional activation through changes in its conformation and interaction with other cellular components. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calmodulin-dependent protein kinases. These kinases can phosphorylate shisa like 2B, leading to its activation through conformational changes that promote its function. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid inhibits protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels on cellular proteins, including shisa like 2B. This can result in the activation of shisa like 2B by maintaining its phosphorylated state. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Similar to okadaic acid, Calyculin A inhibits serine/threonine protein phosphatases, which could lead to the hyperphosphorylation and subsequent activation of shisa like 2B. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG can activate AMP-activated protein kinase (AMPK), which may lead to phosphorylation and activation of downstream proteins, potentially including shisa like 2B, enhancing its activity within its cellular context. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin can act as a JNK (c-Jun N-terminal kinase) activator, which could lead to the phosphorylation of shisa like 2B, potentially activating it by modifying its interaction with other proteins or its stability. | ||||||
Spermidine | 124-20-9 | sc-215900 sc-215900B sc-215900A | 1 g 25 g 5 g | $56.00 $595.00 $173.00 | ||
Spermidine can induce autophagy through activation of AMPK pathway. This process could involve the selective degradation of inhibitors of shisa like 2B, thereby activating shisa like 2B. | ||||||
Resveratrol | 501-36-0 | sc-200808 sc-200808A sc-200808B | 100 mg 500 mg 5 g | $60.00 $185.00 $365.00 | 64 | |
Resveratrol can activate SIRT1, a deacetylase, which can deacetylate and activate various proteins. This deacetylation can modify the function of shisa like 2B, leading to its activation through enhanced protein interactions. | ||||||
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium chloride inhibits GSK-3β (glycogen synthase kinase 3 beta), which can lead to the inactivation of GSK-3β's target proteins' inhibitors, thereby activating those proteins. This can indirectly lead to the activation of shisa like 2B. | ||||||
Sodium Butyrate | 156-54-7 | sc-202341 sc-202341B sc-202341A sc-202341C | 250 mg 5 g 25 g 500 g | $30.00 $46.00 $82.00 $218.00 | 19 | |
Sodium butyrate is a histone deacetylase inhibitor, which can result in a more relaxed chromatin structure and potentially enhanced gene transcription. This could indirectly activate shisa like 2B by promoting the expression of proteins that activate shisa like 2B. | ||||||