F1-ATPase Inhibitors
Chemical inhibitors of F1-ATPase operate through various mechanisms to impede the enzyme's capacity to synthesize ATP. Oligomycin A, for instance, selectively binds to the OSCP subunit of F1-ATPase, effectively halting proton flow through the F0 unit, which is essential for the enzyme's ATP synthesis function. Similarly, Venturicidin A disrupts proton translocation by targeting the F0 component of the ATP synthase complex, thereby inhibiting the enzyme's activity. Bafilomycin A1, while primarily an inhibitor of the vacuolar type H+-ATPase, can indirectly affect F1-ATPase by disrupting the proton gradient. This gradient is crucial for maintaining the acid-base balance and membrane potential necessary for F1-ATPase operation. DCCD, or N,N'-Dicyclohexylcarbodiimide, binds covalently to carboxyl groups on the F0 component's amino acid residues, blocking proton transport and thus ATP synthesis by F1-ATPase.
Continuing with the theme of inhibition, Tributyltin chloride interacts with the protein complex containing F1-ATPase, modifying its conformation and inhibiting ATP synthesis. Quercetin competes with ATP for binding sites on F1-ATPase, hindering the enzyme's catalytic action. Aurovertin B targets the beta subunit, directly inhibiting the enzyme's catalytic activity. Compounds such as Bedaquiline and Efrapeptin also demonstrate inhibitory action; Bedaquiline binds to the c subunit of the F0 complex, interfering with the proton gradient and, consequently, F1-ATPase's functionality, while Efrapeptin prevents the necessary conformational changes within the F1 portion of the enzyme complex. Lastly, agents like Resveratrol and Sodium azide impact F1-ATPase by altering the enzyme's structure or binding to the metal centers crucial for catalysis, thereby impeding the enzyme's ability to produce ATP.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Oligomycin A | 579-13-5 | sc-201551 sc-201551A sc-201551B sc-201551C sc-201551D | 5 mg 25 mg 100 mg 500 mg 1 g | $179.00 $612.00 $1203.00 $5202.00 $9364.00 | 26 | |
Oligomycin A binds to the OSCP (oligomycin sensitivity-conferring protein) subunit of F1-ATPase, specifically inhibiting the proton flow through the F0 unit, which is essential for the ATP synthase activity of F1-ATPase, leading to the functional inhibition of ATP synthesis. | ||||||
Venturicidin A | 33538-71-5 | sc-202380 sc-202380A | 1 mg 5 mg | $207.00 $474.00 | ||
Venturicidin A interferes with the proton translocation through the F0 component of the ATP synthase complex, leading to the inhibition of ATP synthesis by F1-ATPase as the proton gradient is necessary for its function. | ||||||
Bafilomycin A1 | 88899-55-2 | sc-201550 sc-201550A sc-201550B sc-201550C | 100 µg 1 mg 5 mg 10 mg | $98.00 $255.00 $765.00 $1457.00 | 280 | |
Bafilomycin A1 is a specific inhibitor of the vacuolar type H+-ATPase. By disrupting the proton gradient maintained by the V-ATPase, it indirectly inhibits F1-ATPase by altering the general acid-base balance and membrane potential, which are critical for F1-ATPase function. | ||||||
DCC | 538-75-0 | sc-239713 sc-239713A | 25 g 100 g | $72.00 $208.00 | 3 | |
DCCD acts by binding covalently to the carboxyl groups on the amino acid residues of the F0 component of ATP synthase, which is in close interaction with F1-ATPase, inhibiting the enzyme's function by blocking proton transport, which is fundamental for the ATP synthesis carried out by F1-ATPase. | ||||||
TBTC | 1461-22-9 | sc-251105 | 5 g | $48.00 | ||
Tributyltin chloride inhibits the mitochondrial ATPase activity by binding to the protein complex that includes F1-ATPase, affecting its conformation and, therefore, its enzymatic activity which directly prevents ATP synthesis. | ||||||
Diethylstilbestrol | 56-53-1 | sc-204720 sc-204720A sc-204720B sc-204720C sc-204720D | 1 g 5 g 25 g 50 g 100 g | $71.00 $287.00 $547.00 $1098.00 $2185.00 | 3 | |
Diethylstilbestrol, a synthetic nonsteroidal estrogen, has been found to inhibit mitochondrial F1-ATPase activity by interfering with the protein's structure and function, leading to a reduction in ATP synthesis. | ||||||
Quercetin | 117-39-5 | sc-206089 sc-206089A sc-206089E sc-206089C sc-206089D sc-206089B | 100 mg 500 mg 100 g 250 g 1 kg 25 g | $11.00 $17.00 $110.00 $250.00 $936.00 $50.00 | 33 | |
Quercetin can inhibit F1-ATPase by competing with ATP for binding sites on the enzyme, thereby hindering the enzyme's ability to catalyze the synthesis of ATP. | ||||||
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $84.00 $306.00 $1104.00 | 30 | |
Aurovertin B binds to the beta subunit of F1-ATPase, inhibiting its catalytic activity, which results in the inhibition of ATP synthesis. | ||||||
Resveratrol | 501-36-0 | sc-200808 sc-200808A sc-200808B | 100 mg 500 mg 5 g | $80.00 $220.00 $460.00 | 64 | |
Resveratrol has been shown to inhibit the activity of F1-ATPase, possibly by altering the enzyme's structure or the stability of the mitochondrial membrane potential, which is critical for the functioning of ATP synthase. | ||||||
Sodium azide | 26628-22-8 | sc-208393 sc-208393B sc-208393C sc-208393D sc-208393A | 25 g 250 g 1 kg 2.5 kg 100 g | $43.00 $155.00 $393.00 $862.00 $90.00 | 8 | |
Sodium azide inhibits ATP synthesis by binding to the metal centers in the F1-ATPase, particularly those that are essential for the catalytic activity of the enzyme, thereby directly inhibiting its function in ATP production. | ||||||