Cre Recombinase Activators
Cre recombinase activators belong to a specialized category of chemical compounds that are involved in the modulation of the Cre recombinase enzyme, a crucial tool in molecular biology and genetic engineering. Cre recombinase is from the bacteriophage P1 and is widely used in experimental settings to catalyze site-specific recombination between DNA sequences known as loxP sites. When activated by specific compounds, Cre recombinase can efficiently recognize and bind to these loxP sites, leading to the excision, inversion, or translocation of DNA segments flanked by these recognition sequences.
The activation of Cre recombinase by its activators is a fundamental process in genetic manipulation and allows researchers to precisely control the spatial and temporal regulation of gene expression in various organisms. By fine-tuning the activity of Cre recombinase, these activators can influence the efficiency and specificity of DNA recombination events, enabling the creation of genetically modified organisms or cells with a high degree of precision. Understanding the chemical properties and mechanisms underlying Cre recombinase activation is crucial for advancing the field of molecular biology and genetic engineering, as it allows researchers to develop innovative strategies for studying gene function, tracing cellular lineages, and generating animal models with specific genetic modifications. Research in this area contributes to our broader understanding of gene regulation and genetic diversity, facilitating advancements in various scientific disciplines.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Tetracycline | 60-54-8 | sc-205858 sc-205858A sc-205858B sc-205858C sc-205858D | 10 g 25 g 100 g 500 g 1 kg | $63.00 $94.00 $270.00 $417.00 $634.00 | 6 | |
Tetracycline can induce Cre recombinase expression in systems using a tetracycline-responsive promoter. It binds to and activates the tetracycline-controlled transactivator, which then promotes transcription of genes, including Cre. | ||||||
Doxycycline-d6 | 564-25-0 unlabeled | sc-218274 | 1 mg | $16500.00 | ||
Similar to tetracycline, doxycycline can activate a tetracycline-responsive promoter. It's often used due to its more stable and effective profile in inducing gene expression. | ||||||
Tamoxifen | 10540-29-1 | sc-208414 | 2.5 g | $272.00 | 18 | |
Tamoxifen induces Cre recombinase expression in systems where Cre is fused to a modified estrogen receptor. The compound binds to this receptor, allowing Cre to translocate to the nucleus and become active. | ||||||
Mifepristone | 84371-65-3 | sc-203134 | 100 mg | $61.00 | 17 | |
Mifepristone can activate promoters that are responsive to glucocorticoids or progestins. When Cre is placed under such promoters, mifepristone can induce its expression. | ||||||
Dexamethasone | 50-02-2 | sc-29059 sc-29059B sc-29059A | 100 mg 1 g 5 g | $91.00 $139.00 $374.00 | 36 | |
Dexamethasone is a glucocorticoid that can induce Cre recombinase expression when Cre is under the control of glucocorticoid-responsive promoters. | ||||||
IPTG, Dioxane-Free | 367-93-1 | sc-202185 sc-202185A sc-202185B sc-202185C sc-202185D sc-202185E sc-202185F | 1 g 5 g 100 g 500 g 1 kg 10 kg 25 kg | $51.00 $117.00 $510.00 $1785.00 $2240.00 $16646.00 $32773.00 | 27 | |
IPTG induces Cre recombinase expression in systems using a lac operon-based promoter. It binds to and inactivates the lac repressor, allowing transcription of the Cre gene. | ||||||
(Z)-4-Hydroxytamoxifen | 68047-06-3 | sc-3542C sc-3542 sc-3542B sc-3542A sc-3542D sc-3542E | 1 mg 5 mg 10 mg 25 mg 50 mg 100 mg | $220.00 $281.00 $408.00 $718.00 $1377.00 $2397.00 | 20 | |
(Z)-4-Hydroxytamoxifen is an active metabolite of tamoxifen and functions similarly by binding to estrogen receptor-based fusion proteins to induce Cre expression. | ||||||
Acyclovir | 59277-89-3 | sc-202906 sc-202906A | 50 mg 500 mg | $150.00 $940.00 | 2 | |
Acyclovir can be used to induce Cre recombinase expression in systems where the promoter is responsive to nucleoside analogs. | ||||||
Gibberellic acid | 77-06-5 | sc-257556 sc-257556A sc-257556B sc-257556C | 500 mg 1 g 5 g 25 g | $47.00 $63.00 $145.00 $515.00 | 2 | |
In plant systems, gibberellic acid can induce Cre recombinase expression when Cre is under the control of a gibberellin-responsive promoter. | ||||||
3-Indoleacetic acid | 87-51-4 | sc-254494 sc-254494A sc-254494B | 5 g 25 g 100 g | $31.00 $92.00 $160.00 | 4 | |
This is a plant hormone that can induce the expression of Cre recombinase in systems using an auxin-responsive promoter in plants. | ||||||