AKAP 100 Inhibitors
Chemical inhibitors of AKAP 100 act by disrupting the protein's ability to interact with key signaling molecules within the cell. H-89, for instance, inhibits AKAP 100 by preventing its association with protein kinase A (PKA), a critical kinase for numerous cellular processes. By blocking the PKA binding domain, H-89 disrupts the AKAP 100-PKA signaling complex. Similarly, Rp-cAMPs and Rp-8-Br-cAMPS work by competing with cyclic AMP (cAMP) for PKA binding, preventing the formation of active AKAP 100-PKA complexes. PKI 14-22 amide, myristoylated directly inhibits the catalytic activity of PKA, which is necessary for AKAP 100 to anchor and position PKA for proper cellular function. KT5720 and St-Ht31 also disrupt PKA activity, with KT5720 directly inhibiting the kinase and St-Ht31 interfering with the interaction between PKA and AKAPs, including AKAP 100.
Further inhibitory effects come from compounds that affect other kinases and signaling pathways that AKAP 100 is likely involved in. ML-7, by inhibiting myosin light chain kinase, can influence the actin cytoskeleton and cellular contractility, processes which AKAP 100 may help regulate through the spatial organization of signaling complexes. Go6976 and Bisindolylmaleimide I, both inhibitors of protein kinase C (PKC), can indirectly inhibit AKAP 100 by altering PKC-mediated pathways and preventing the assembly of AKAP 100-associated signaling domains. In the realm of calcium signaling, KN-93 and Autocamtide-2-related inhibitory peptide target calcium/calmodulin-dependent protein kinase II (CaMKII), a kinase that AKAP 100 may scaffold. By inhibiting CaMKII, these compounds prevent AKAP 100 from fulfilling its role in localizing CaMKII activity within the cell. Lastly, GSK690693 inhibits AKT kinase activity. As AKAP 100 may act to position AKT within specific signaling complexes, GSK690693's inhibition of AKT thus indirectly impedes the downstream signaling events facilitated by AKAP 100.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
H-89 dihydrochloride | 130964-39-5 | sc-3537 sc-3537A | 1 mg 10 mg | $94.00 $186.00 | 71 | |
H-89 is a protein kinase A (PKA) inhibitor that can inhibit AKAP 100 by preventing its association with PKA, thereby disrupting the AKAP 100-PKA signaling complex critical for localized PKA signaling. | ||||||
Rp-cAMPS | 151837-09-1 | sc-24010 | 1 mg | $203.00 | 37 | |
Rp-cAMPs is a PKA inhibitory cyclic AMP analog that can inhibit AKAP 100 by competing with cAMP for PKA binding, thus preventing AKAP 100 from anchoring active PKA in specific cellular locations. | ||||||
PKI (14-22) amide (myristoylated) | 201422-03-9 | sc-471154 | 0.5 mg | $135.00 | 2 | |
PKI 14-22 amide, myristoylated is a specific PKA inhibitor peptide that can inhibit AKAP 100 by blocking PKA's catalytic subunit, which is necessary for AKAP 100 to anchor PKA and facilitate its cellular functions. | ||||||
KT 5720 | 108068-98-0 | sc-3538 sc-3538A sc-3538B | 50 µg 100 µg 500 µg | $138.00 $220.00 $972.00 | 47 | |
KT5720 is a PKA inhibitor that can inhibit AKAP 100 by blocking PKA activity, thus preventing the AKAP 100 from serving as a scaffolding protein for PKA-mediated phosphorylation events. | ||||||
ML-7 hydrochloride | 110448-33-4 | sc-200557 sc-200557A | 10 mg 50 mg | $91.00 $267.00 | 13 | |
ML-7 is a myosin light chain kinase inhibitor that can inhibit AKAP 100 indirectly by affecting the contractility and cytoskeletal dynamics that AKAP 100 may be involved in regulating through spatial organization of signaling molecules. | ||||||
Rp-8-Br-cAMPS | 129735-00-8 | sc-3539A sc-3539 | 500 µg 1 mg | $250.00 $340.00 | 22 | |
Rp-8-Br-cAMPS is a PKA inhibitory cyclic AMP analog that can inhibit AKAP 100 by blocking PKA activation and thus preventing AKAP 100 from localizing PKA to specific intracellular locations for signaling. | ||||||
Gö 6976 | 136194-77-9 | sc-221684 | 500 µg | $227.00 | 8 | |
Go6976 is a protein kinase C (PKC) inhibitor that can inhibit AKAP 100 indirectly by altering PKC-mediated pathways and signaling events that AKAP 100 may influence through scaffolding of PKC with other signaling proteins. | ||||||
KN-93 | 139298-40-1 | sc-202199 | 1 mg | $182.00 | 25 | |
KN-93 is a calcium/calmodulin-dependent protein kinase II (CaMKII) inhibitor that can inhibit AKAP 100 by disrupting CaMKII signaling which AKAP 100 may facilitate through targeting of CaMKII to specific cellular compartments. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I is another PKC inhibitor that can inhibit AKAP 100 indirectly by impeding PKC activity, which AKAP 100 may modulate by organizing PKC within cellular microdomains. | ||||||
GSK 690693 | 937174-76-0 | sc-363280 sc-363280A | 10 mg 50 mg | $255.00 $1071.00 | 4 | |
GSK690693 is an Akt inhibitor that can inhibit AKAP 100 indirectly by inhibiting AKT kinase activity, which AKAP 100 might scaffold to specific signaling complexes, hence affecting downstream signaling that requires AKAP 100 facilitation. | ||||||