Zwilch Antibody (NB-C38): sc-135615
- Zwilch Antibody (NB-C38) is a mouse monoclonal IgG1 κ Zwilch antibody provided at 100 µg/ml
- raised against recombinant Zwilch protein of human origin
- recommended for detection of Zwilch of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Zwilch Antibody (NB-C38). This work is in progress.
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Zwilch Antibody (NB-C38) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Zwilch protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Zwilch antibody (NB-C38) is available as the non-conjugated format. Zwilch (NB-C38) antibody recognizes a protein that plays a critical role in regulating chromosome segregation during cell division, essential for maintaining genomic stability. Zwilch (NB-C38) monoclonal antibody targets a protein that interacts with ROD (Rough Deal) and ZWINT (Zeste-White 10) to form a complex vital for recruiting cytoplasmic Dynein to the kinetochore, ensuring proper spindle checkpoint function. This interaction remains evolutionarily conserved, as demonstrated by co-immunoprecipitation (IP) of human Zwilch, ZWINT, and ROD in HeLa cells, highlighting the complex′s importance in human cellular processes. Localization of Zwilch, ZWINT, and ROD complex to kinetochores during prometaphase underscores their significance in accurate chromosome segregation. Mutations in Zwilch, ZWINT, and ROD identified in colorectal cancer suggest that alterations in this complex may contribute to chromosomal instability, a hallmark of many cancers. Anti-Zwilch antibody (NB-C38) effectively detects this crucial protein, making this reagent valuable for researchers studying cell division and cancer biology.
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Zwilch Antibody (NB-C38) References:
- Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions. | Williams, BC., et al. 2003. Mol Biol Cell. 14: 1379-91. PMID: 12686595
- Three classes of genes mutated in colorectal cancers with chromosomal instability. | Wang, Z., et al. 2004. Cancer Res. 64: 2998-3001. PMID: 15126332
- ZW10 links mitotic checkpoint signaling to the structural kinetochore. | Kops, GJ., et al. 2005. J Cell Biol. 169: 49-60. PMID: 15824131
- Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex. | Buffin, E., et al. 2005. Curr Biol. 15: 856-61. PMID: 15886105
- Rod-Zw10-Zwilch: a key player in the spindle checkpoint. | Karess, R. 2005. Trends Cell Biol. 15: 386-92. PMID: 15922598
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Zwilch Antibody (NB-C38) | sc-135615 | 100 µg/ml | $339.00 |