Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZIP-kinase Antibody (C-3): sc-514223. Western blot analysis of ZIP-kinase expression in HeLa (A), K-562 (B) and HCT-116 (C) whole cell lysates. Detection reagent used: m-IgG1 BP-HRP: sc-525408.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZIP-kinase CRISPR/Cas9 KO Plasmid (h): sc-409173
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZIP-kinase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZIP-kinase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZIP-kinase Antibody (C-3): sc-514223
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZIP-kinase CRISPR/Cas9 KO Plasmid (h) | sc-409173 | 20 µg | $397.00 |