Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZIP-kinase CRISPR/Cas9 KO Plasmid (h): sc-409173
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZIP-kinase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZIP-kinase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZIP-kinase Antibody (C-3): sc-514223
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZIP-kinase CRISPR/Cas9 KO Plasmid (h) | sc-409173 | 20 µg | $397.00 |
Overview
DAPK3 (ZIP-kinase) is a Ca2+/calmodulin-regulated serine/threonine kinase that coordinates actomyosin dynamics and contractile signaling through phosphorylation of myosin regulatory light chain and related cytoskeletal substrates. It participates in pathways controlling stress fiber formation, membrane blebbing, cytokinesis, and programmed cell death, linking cytoskeletal remodeling to cellular stress responses. ZIP-kinase activity intersects with ROCK/MLCK-regulated contractility and can influence transcriptional outputs via phosphorylation-dependent signaling nodes. Dysregulated DAPK3 signaling has been associated with altered proliferation, motility, and apoptosis phenotypes relevant to cancer biology, as well as vascular and inflammatory disease mechanisms where smooth muscle tone and cytoskeletal organization are perturbed.
ZIP-kinase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DAPK3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DAPK3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DAPK3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZIP-kinase protein expression.
This CRISPR knockout system enables efficient generation of DAPK3-deficient cell models for investigation of ZIP-kinase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DAPK3 exon(s) critical for ZIP-kinase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DAPK3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZIP-kinase CRISPR/Cas9 KO Plasmid (h) and ZIP-kinase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DAPK3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZIP-kinase HDR Plasmid (h) and ZIP-kinase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DAPK3 homology arms to support homology-directed repair at defined DAPK3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.