Date published: 2025-9-7
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YB-1 Antibody (C-3) - Western Blotting - Image 388025
YB-1 抗体 (C-3): sc-398340. HeLa (A), K-562 (B) 和 Jurkat (C) 全细胞裂解液中 YB-1 表达的 Western 印迹分析.
YB-1 抗体 (C-3): sc-398340. HeLa (A), K-562 (B) 和 Jurkat (C) 全细胞裂解液中 YB-1 表达的 Western 印迹分析.

YB-1 抗体 (C-3): sc-398340

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寫評論提問

说明书
  • YB-1 抗体 C-3 是小鼠单克隆 IgG2a κ，YB-1抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列196-240位于human物种的YB-1的内部区域内
  • YB-1 抗体 (C-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 YB-1
  • 抗YB-1抗体（C-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-398340 X, 200 µg/0.1 ml)
  • 关于如何获取YB-1 (C-3): sc-398340的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 YB-1 抗体 (C-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与YB-1 抗体 (C-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

YB-1抗体（C-3）是一种IgG2a κ小鼠单克隆YB-1抗体（也称为YBOX1抗体、Y-Box结合蛋白1抗体、DBPB抗体、YB1抗体、CCAAT结合转录因子I亚基A抗体、核酸酶敏感元素结合蛋白1抗体、增强子因子I亚基A抗体、Y-Box转录因子抗体、Y-Box结合蛋白1抗体、DNA结合蛋白B抗体、CBF-A抗体、CSDA2抗体、EFI-A抗体、NSEP1抗体、BP-8抗体或CSDB抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的YB-1蛋白。YB-1抗体（C-3）可作为非偶联的抗YB-1抗体形式提供，也可作为多种偶联形式的抗YB-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Y-box结合蛋白YB-1（也称为CCAAT结合转录因子、增强子因子I亚基A和DNA结合蛋白B）属于一类多功能蛋白质家族，这类蛋白质同时调节转录和翻译过程。Y-box蛋白能与多种核酸结构相互作用，发挥转录因子和mRNA掩蔽蛋白的作用。Y-box蛋白的模块化结构包括一个高度保守的N端冷休克结构域（CSD，相当于细菌冷休克蛋白）和四个包含精氨酸簇和芳香族残基的基本C端结构域。YB-1作为生长相关基因表达的激活剂，在细胞增殖中发挥作用。同时，YB-1也是细胞死亡相关基因FAS的抑制因子。因此，YB-1可能通过调节细胞生长相关和死亡相关基因的表达，在控制细胞存活中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

YB-1 抗体 (C-3) 参考文献:

  1. YB-1在40S核糖体亚基结合步骤或之前，对其自身mRNA的翻译进行自我调节。  |  Skabkina, OV., et al. 2005. Mol Cell Biol. 25: 3317-23. PMID: 15798215
  2. YB-1和MTA1蛋白水平而非DNA或mRNA改变可预测前列腺癌复发。  |  Sheridan, CM., et al. 2015. Oncotarget. 6: 7470-80. PMID: 25797255
  3. 转运蛋白1依赖性YB-1核内转运。  |  Mordovkina, DA., et al. 2016. Biochem Biophys Res Commun. 480: 629-634. PMID: 27794479
  4. YB-3在全球mRNA结合中替代YB-1。  |  Lyabin, DN., et al. 2020. RNA Biol. 17: 487-499. PMID: 31944153
  5. YB1通过抑制肝癌中microRNA的成熟来调节miR-205/200b-ZEB1轴。  |  Liu, X., et al. 2021. Cancer Commun (Lond). 41: 576-595. PMID: 34110104
  6. 对YB-1在癌症干细胞、转移和耐药性中的功能的全面综述。  |  Alkrekshi, A., et al. 2021. Cell Signal. 85: 110073. PMID: 34224843
  7. 对YB-1信号传导和细胞死亡决策的新见解。  |  Shah, A., et al. 2021. Cancers (Basel). 13: PMID: 34282755
  8. YB-1的丝氨酸209位磷酸化抑制其核易位。  |  Sogorina, EM., et al. 2021. Int J Mol Sci. 23: PMID: 35008856
  9. YB-1激活级联作为KRAS突变肿瘤的潜在靶标。  |  Khozooei, S., et al. 2023. Strahlenther Onkol. 199: 1110-1127. PMID: 37268766
  10. ABA通过NF-YB1-SLRL2-bHLH144模块调节水稻谷粒质量和种子休眠。  |  Wang, JD., et al. 2024. Nat Commun. 15: 4493. PMID: 38802342

订购信息

产品名称产品编号规格价格数量收藏夹

YB-1 抗体 (C-3)

sc-398340
200 µg/ml
$316.00

YB-1 (C-3): m-IgG Fc BP-HRP 套装

sc-530765
200 µg Ab; 10 µg BP
$354.00

YB-1 (C-3): m-IgGκ BP-HRP 套装

sc-524300
200 µg Ab, 40 µg BP
$354.00

YB-1 (C-3): m-IgG2a BP-HRP 套装

sc-547582
200 µg Ab; 10 µg BP
$354.00

YB-1 抗体 (C-3) X

sc-398340 X
200 µg/0.1 ml
$316.00

YB-1 抗体 (C-3) AC

sc-398340 AC
500 µg/ml, 25% agarose
$416.00

YB-1 抗体 (C-3) HRP

sc-398340 HRP
200 µg/ml
$316.00

YB-1 抗体 (C-3) FITC

sc-398340 FITC
200 µg/ml
$330.00

YB-1 抗体 (C-3) PE

sc-398340 PE
200 µg/ml
$343.00

YB-1 抗体 (C-3) Alexa Fluor® 488

sc-398340 AF488
200 µg/ml
$357.00

YB-1 抗体 (C-3) Alexa Fluor® 546

sc-398340 AF546
200 µg/ml
$357.00

YB-1 抗体 (C-3) Alexa Fluor® 594

sc-398340 AF594
200 µg/ml
$357.00

YB-1 抗体 (C-3) Alexa Fluor® 647

sc-398340 AF647
200 µg/ml
$357.00

YB-1 抗体 (C-3) Alexa Fluor® 680

sc-398340 AF680
200 µg/ml
$357.00

YB-1 抗体 (C-3) Alexa Fluor® 790

sc-398340 AF790
200 µg/ml
$357.00

I cloned the human YBX1 CCDS470 (NM_004559.5) into a pCMV-HA vector. Unfortunately the antibody YB-1 (C-3): sc-398340 is not recognizing the HA-YB1 after transfection in 293T cells. Can you please confirm that this antibody should recognize this form?

Asked by: elob
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-02-09

I am using YB-1 (C-3): sc-398340 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice antibodyWorks well in Western Blot analyses of human cell lysates (dilution 1:500 to 1:1000).
Date published: 2023-11-30
Rated 4 out of 5 by from YB1 WesternsWorks reasonably well for westerns 1:100 dilution Hela cells, overnight incubation at 4 Degrees
Date published: 2017-11-29
Rated 5 out of 5 by from Excellent antibodyWe got this antibody a month ago and have used for western blotting and IHC. It is highly sensitive and shows no background. Highly recommended!
Date published: 2017-10-19
Rated 4 out of 5 by from excellent antibody to detect human Yb-1Great antibody with clear and single band for detecting Yb-1 in hcc cells.
Date published: 2017-09-19
Rated 5 out of 5 by from WELL FOR WBIT WORKED WELL BY WB IN LAB, WILL PURCHASE IT IN THE FUTURE
Date published: 2017-02-20
Rated 4 out of 5 by from Good staining for western blotThis antibody is good for western blot. I will try on IHC in the future
Date published: 2017-02-14
Rated 5 out of 5 by from Good choice for IHC(P) and WBGreat staining, clear and specific. Western data is clean with no background or non-specifics.
Date published: 2017-01-20
Rated 5 out of 5 by from Very good Western blot data of YBVery good Western blot data of YB-1 expression in MCF7 and SW480 whole cell lysates. -SCBT QC
Date published: 2014-10-08
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YB-1 抗体 (C-3) is rated 4.6 out of 5 by 8.
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