Date published: 2025-9-9
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YB-1 Antibody (59-Q) - Western Blotting - Image 21991
YB-1 抗体 (59-Q): sc-101198. 未转染 293T: sc-117752 (A), 转染人 YB-1 的 293T: sc-113221 (B) 和 HeLa (C) 全细胞裂解液中 YB-1 表达的 Western 印迹分析.
YB-1 抗体 (59-Q): sc-101198. 未转染 293T: sc-117752 (A), 转染人 YB-1 的 293T: sc-113221 (B) 和 HeLa (C) 全细胞裂解液中 YB-1 表达的 Western 印迹分析.

YB-1 抗体 (59-Q): sc-101198

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说明书
  • YB-1抗体(59-Q)是小鼠单克隆IgG2a κ, 在34篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组YB-1
  • 推荐用于 mouse, rat 和 human 来源的YB-1 WB, IP, IF 和 ELISA检测
  • 关于如何获取YB-1 (59-Q): sc-101198的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是YB-1 Antibody (59-Q) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与YB-1 Antibody (59-Q) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

YB-1抗体（59-Q）是一种IgG2a κ小鼠单克隆YB-1抗体（也称为YB-1抗体或YBX1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的YB-1。YB-1抗体（59-Q）可作为非偶联的抗YB-1抗体形式提供。Y-box结合蛋白YB-1（也称为CCAAT结合转录因子、增强子因子I亚基A和DNA结合蛋白B）属于一类多功能蛋白质家族，这类蛋白质同时调节转录和翻译过程。Y-box蛋白能与多种核酸结构相互作用，发挥转录因子和mRNA掩蔽蛋白的作用。Y-box蛋白的模块化结构包括一个高度保守的N端冷休克结构域（CSD，相当于细菌冷休克蛋白）和四个包含精氨酸簇和芳香族残基的基本C端结构域。YB-1作为生长相关基因表达的激活剂，在细胞增殖中发挥作用。同时，YB-1也是细胞死亡相关基因FAS的抑制因子。因此，YB-1可能通过调节细胞生长相关和死亡相关基因的表达，在控制细胞存活中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

YB-1 抗体 (59-Q) 参考文献:

  1. YB-1, Puralpha 和 AP-1 转录因子对人类 fas 启动子的调控。  |  Lasham, A., et al. 2000. Gene. 252: 1-13. PMID: 10903433
  2. 对 DNA 交互作用药物的多基因抗性与参与 DNA 复制, 修复和应激反应的基因表达增加有关。  |  Levenson, VV., et al. 2000. Cancer Res. 60: 5027-30. PMID: 11016623
  3. 大肠杆菌 CspA 与人类 YB-1 冷休克结构域杂化体获得双链 DNA 结合能力。  |  Wang, N., et al. 2000. Mol Microbiol. 38: 526-34. PMID: 11069676
  4. 冷休克结构域因子可激活受刺激的 Jurkat T 细胞中的粒细胞-巨噬细胞集落刺激因子启动子。  |  Diamond, P., et al. 2001. J Biol Chem. 276: 7943-51. PMID: 11116154
  5. p53 与 Y-box 结合蛋白 YB-1 的直接相互作用：人类基因表达的调控机制。  |  Okamoto, T., et al. 2000. Oncogene. 19: 6194-202. PMID: 11175333
  6. 致癌 TLS/ERG 和 EWS/Fli-1 融合蛋白会抑制 YB-1 蛋白介导的 RNA 剪接。  |  Chansky, HA., et al. 2001. Cancer Res. 61: 3586-90. PMID: 11325824
  7. Y-盒结合蛋白1--预后标志和肿瘤治疗靶点。  |  Kosnopfel, C., et al. 2014. Eur J Cell Biol. 93: 61-70. PMID: 24461929
  8. 将抑制 Y 盒结合蛋白 1 作为卵巢癌的靶向疗法。  |  Tailor, D., et al. 2021. Cell Chem Biol. 28: 1206-1220.e6. PMID: 33713600
  9. 以 Y-box 结合蛋白-1 轴为靶点克服实体瘤的放化疗抗药性  |  Lettau, K., et al. 2021. Int J Radiat Oncol Biol Phys. 111: 1072-1087. PMID: 34166770
  10. YB-1是基底样乳腺癌中KLF5转录因子的正向调节因子。  |  Jiang, D., et al. 2022. Cell Death Differ. 29: 1283-1295. PMID: 35022570
  11. 上调的YB-1蛋白通过YB-1/CCT4/mLST8/mTOR途径促进胶质母细胞瘤的生长。  |  Wang, JZ., et al. 2022. J Clin Invest. 132: PMID: 35239512
  12. 敲除 Y-box 结合蛋白 1 可诱导猪早期胚胎的自噬。  |  Jiang, WJ., et al. 2023. Front Cell Dev Biol. 11: 1238546. PMID: 37965572

订购信息

产品名称产品编号规格价格数量收藏夹

YB-1 抗体 (59-Q)

sc-101198
100 µg/ml
$333.00

YB-1 (59-Q): m-IgG Fc BP-HRP 套装

sc-537304
100 µg Ab; 10 µg BP
$354.00

YB-1 (59-Q): m-IgGκ BP-HRP 套装

sc-534537
100 µg Ab; 40 µg BP
$354.00

YB-1 (59-Q): m-IgG2a BP-HRP 套装

sc-547974
100 µg Ab; 10 µg BP
$354.00

Can this antibody also be used for a surface staining of living cells in Flow cytometry? Thanks in advance!

Asked by: Nina
Thank you for your question. YB-1 Antibody (59-Q): sc-101198 is only recommended for detection of YB-1 of mouse, rat and human origin by WB, IP, IF and ELISA. We do not have data to support use in flow cytometry assays.
Answered by: Tech Support Europe
Date published: 2025-03-26

Can you please tell me the exact immunogen peptide for generation of YB-1 Antibody (59-Q): sc-101198? Thank you in advance.

Asked by: Mel12
Thank you for your question. This antibody was raised against a recombinant protein mapping within amino acids 51-140 of YB-1 of human origin. The exact epitope has not been mapped, so no further information is available.
Answered by: Tech Service
Date published: 2023-01-27

What is the total volume provided of YB-1 (59-Q): sc-101198?

Asked by: jenni
Each vial contains 100 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Service 11
Date published: 2017-01-17

What is the recommended fixation method for IF with YB-1 (59-Q): sc-101198 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-01-16
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Rated 5 out of 5 by from Great AbWorking good in casein blocking buffer (1:500, 100ug/ml); 40ug of protein loaded. Detected 50kDa (major band) and >30kDa (weak and cell line dependent)
Date published: 2018-01-03
Rated 5 out of 5 by from Excellent Antibody for WB.Work well in WB at 1:1000, Hep-1 cells. Strong and clear band was observed.
Date published: 2017-04-16
Rated 5 out of 5 by from Strong western and IFVery nice antibody for western blot studies; also strong IF staining
Date published: 2017-01-24
Rated 5 out of 5 by from Really strong antibody!Great antibody for IHC and WB! It's reliable and specific, so it's a great choice if you're looking for a YB-1 antibody.
Date published: 2017-01-16
Rated 5 out of 5 by from Good signal with IF and WBI got exceptional immunofluorescence cytoplasmic staining as well as western blot in NIH3T3 cells
Date published: 2016-09-09
Rated 4 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-10-29
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-07-13
Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclearProduced exceptional immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-09
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YB-1 抗体 (59-Q) is rated 4.7 out of 5 by 9.
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