YAP (h): 293T Lysate: sc-115429

YAP (h): 293T Lysate originates from the human embryonic kidney 293T cell line engineered to express the Yes-associated protein (YAP), a key downstream effector of the Hippo signaling pathway. YAP is a transcriptional co-activator that regulates gene expression by interacting with transcription factors such as TEADs (TEA domain family members) to promote cell proliferation, survival, and organ size control. The 293T cell line is widely used in research due to its high transfection efficiency and robust protein expression capabilities, making it suitable for generating YAP lysates. In scientific investigations, YAP (h): 293T Lysate serves as a valuable tool for studying the molecular mechanisms underlying the Hippo signaling pathway and its role in various cellular processes. Researchers utilize this lysate in biochemical assays such as immunoprecipitation, Western blotting, and chromatin immunoprecipitation to analyze YAP expression, post-translational modifications, and interactions with other proteins within the Hippo pathway. Additionally, YAP (h): 293T Lysate is employed in functional studies to explain the physiological functions of YAP in cellular proliferation, differentiation, and tissue regeneration. By utilizing this lysate, researchers can advance their understanding of Hippo signaling and its implications in development, organogenesis, and cancer biology.