Xanthine Oxidase Double Nickase Plasmid (h): sc-401185-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Xanthine Oxidase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Xanthine Oxidase Double Nickase Plasmid (h) and Xanthine Oxidase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XDH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Xanthine Oxidase Antibody (A-3): sc-398548
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Xanthine Oxidase Double Nickase Plasmid (h) | sc-401185-NIC | 20 µg | $410.00 | |||
Xanthine Oxidase Double Nickase Plasmid (h2) | sc-401185-NIC-2 | 20 µg | $410.00 |
XDH encodes xanthine oxidase, a molybdenum-containing oxidoreductase that catalyzes the terminal steps of purine catabolism by converting hypoxanthine to xanthine and xanthine to uric acid. During these reactions the enzyme can generate reactive oxygen species, linking XDH activity to redox homeostasis, oxidative stress signaling, and inflammatory responses. Xanthine oxidase function intersects with metabolic adaptation pathways and tissue injury processes where purine turnover and oxidant production are elevated. Dysregulated XDH expression or activity has been associated with hyperuricemia-related biology, endothelial dysfunction, and oxidative damage in cardiometabolic and inflammatory disease contexts, supporting its use as a mechanistic target in metabolism and stress-response research.
Xanthine Oxidase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XDH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XDH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XDH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XDH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.