Date published: 2025-11-4
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Wnt-5a Antibody (A-5) - Western Blotting - Image 139170
Wnt-5a 抗体 (A-5): sc-365370. 全长人重组 Wnt-5a 融合蛋白的 Western 印迹分析.
Wnt-5a Antibody (A-5) - Western Blotting - Image 139170
Wnt-5a 抗体 (A-5): sc-365370. 全长人重组 Wnt-5a 融合蛋白的 Western 印迹分析.
Wnt-5a 抗体 (A-5): sc-365370. 全长人重组 Wnt-5a 融合蛋白的 Western 印迹分析.

Wnt-5a 抗体 (A-5): sc-365370

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说明书
  • Wnt-5a 抗体 A-5 是小鼠单克隆 IgG2b κ，Wnt-5a抗体, 在50篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列23-80位于human物种的Wnt-5a的N-terminus附近
  • Wnt-5a 抗体 (A-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Wnt-5a
  • 抗Wnt-5a抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 目前，我们还没有完成Wnt-5a 抗体 (A-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Wnt抗体，包括Wnt-1, Wnt-10a, Wnt-10b, Wnt-11, Wnt-16, Wnt-2, Wnt-2b, Wnt-3, Wnt-3a, Wnt-4, Wnt-5a, Wnt-5b, Wnt-7a, Wnt-7b和Wnt-8a

Wnt-5a抗体（A-5）是一种IgG2b κ小鼠单克隆Wnt-5a抗体（也被称作WNT5A抗体），能够通过Western Blot（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测到小鼠、大鼠和人类的Wnt-5a蛋白。Wnt-5a抗体（A-5）既可以作为非偶联的抗Wnt-5a抗体形式提供，也可以作为多种偶联形式的抗Wnt-5a抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。Wnt基因属于一个原癌基因家族，已知至少有13个成员，在从果蝇到人类的物种中都有表达。Wnt这个名字表示这个家族与果蝇的节段极性基因"wingless"及其脊椎动物直系同源物Int1（一个小鼠原癌基因）之间的关系。Wnt家族基因的转录似乎受到精确的时间和空间方式的发育调控。Wnt基因编码富含半胱氨酸的假定糖蛋白，这些糖蛋白具有分泌生长因子的典型特征。通过Northern印迹分析检测到Wnt-5a在脑、肺和心脏中的表达。观察到至少五种不同的Wnt-5a转录本，这是由于起始甲硫氨酸5'端的转录变异性所致。原位杂交在小鼠中检测到Wnt-5a的复杂空间和时间模式，包括在发育中的中枢神经系统、肢体、面部过程和胎儿后部的表达。人类frizzled-5是Wnt-5a配体的受体。有研究表明，Wnt-5a在体内增强了原始造血发育，并代表了人体内造血干细胞功能的体内调节因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Wnt-5a 抗体 (A-5) 参考文献:

  1. Wnt-5A 可增强人体造血干细胞在体内的再繁殖能力和原始造血发育。  |  Murdoch, B., et al. 2003. Proc Natl Acad Sci U S A. 100: 3422-7. PMID: 12626754
  2. 恶性人类神经母细胞中的Wnt-5a基因表达。  |  Blanc, E., et al. 2005. Cancer Lett. 228: 117-23. PMID: 15925444
  3. Wnt-5a是一种突触生成因子，具有对抗Aβ毒性的神经保护特性。  |  Varela-Nallar, L., et al. 2012. Neurodegener Dis. 10: 23-6. PMID: 22261402
  4. 小鼠胎儿和成年发育过程中多种新型 Wnt-1/int-1 相关基因的表达。  |  Gavin, BJ., et al. 1990. Genes Dev. 4: 2319-32. PMID: 2279700
  5. WNT-5A：健康和疾病中的信号和功能。  |  Kumawat, K. and Gosens, R. 2016. Cell Mol Life Sci. 73: 567-87. PMID: 26514730
  6. WNT-5A可调节肝纤维化中TGF-β的相关活动。  |  Beljaars, L., et al. 2017. Am J Physiol Gastrointest Liver Physiol. 312: G219-G227. PMID: 28057611
  7. 间充质WNT-5A/5B信号抑制肺泡上皮祖细胞。  |  Wu, X., et al. 2019. Cells. 8: PMID: 31557955
  8. Wnt-5A/B信号在终生的造血中的作用。  |  Mastelaro de Rezende, M., et al. 2020. Cells. 9: PMID: 32751131
  9. Wnt-5a可诱导海马神经元从沉默突触转变为功能性突触。  |  Álvarez-Ferradas, C., et al. 2022. Front Mol Neurosci. 15: 1024034. PMID: 36385757
  10. 逆转非典型Wnt-5a的双重表观遗传抑制，使糖尿病角膜上皮伤口愈合和干细胞恢复正常。  |  Shah, R., et al. 2023. Diabetologia. 66: 1943-1958. PMID: 37460827
  11. 人类原癌基因 Wnt-5A 的分子克隆及该基因（WNT5A）在染色体 3p14-p21 上的图谱。  |  Clark, CC., et al. 1993. Genomics. 18: 249-60. PMID: 8288227
  12. Frizzled 蛋白家族的一个成员，通过 Wnt-5A 介导轴诱导。  |  He, X., et al. 1997. Science. 275: 1652-4. PMID: 9054360

订购信息

产品名称产品编号规格价格数量收藏夹

Wnt-5a 抗体 (A-5)

sc-365370
200 µg/ml
$316.00

Wnt-5a 抗体 (A-5) AC

sc-365370 AC
500 µg/ml, 25% agarose
$416.00

Wnt-5a 抗体 (A-5) HRP

sc-365370 HRP
200 µg/ml
$316.00

Wnt-5a 抗体 (A-5) FITC

sc-365370 FITC
200 µg/ml
$330.00

Wnt-5a 抗体 (A-5) PE

sc-365370 PE
200 µg/ml
$343.00

Wnt-5a 抗体 (A-5) Alexa Fluor® 488

sc-365370 AF488
200 µg/ml
$357.00

Wnt-5a 抗体 (A-5) Alexa Fluor® 546

sc-365370 AF546
200 µg/ml
$357.00

Wnt-5a 抗体 (A-5) Alexa Fluor® 594

sc-365370 AF594
200 µg/ml
$357.00

Wnt-5a 抗体 (A-5) Alexa Fluor® 647

sc-365370 AF647
200 µg/ml
$357.00

Wnt-5a 抗体 (A-5) Alexa Fluor® 680

sc-365370 AF680
200 µg/ml
$357.00

Wnt-5a 抗体 (A-5) Alexa Fluor® 790

sc-365370 AF790
200 µg/ml
$357.00

Why the molecular weight of mouse tissue is 65kD, not 40KD

Asked by: yaqian feng
Thank you for your question. The expected molecular weight for Wnt-5a is 39kDa. The image on our website for this product is of a tagged fusion protein, and therefore is not going to give a band at the endogenous protein size.
Answered by: Tech Service
Date published: 2019-12-02

WHy is this a replacement for the H58 Rabbit polyclonal antibody? Can I get a sample to test?

Asked by: kathyevans01
Thank you for your question. The rabbit polyclonal antibody, Wnt-5a (H-58): sc-30224, is now discontinued. This mouse monoclonal antibody, Wnt-5a (A-5): sc-365370, is raised against the same recombinant protein immunogen as the rabbit polyclonal antibody, corresponding to amino acids 23-80 mapping near the N-terminus of Wnt-5a of human origin. This monoclonal antibody is also recommended for detection of Wnt-5a of mouse, rat and human origin by WB, IP, IF and ELISA. For a limited time, we are offering a free 10 µg sample of this antibody for testing. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-29

Can Wnt-5a (A-5): sc-365370 monoclonal antibody detect endogenous Wnt-5a?

Asked by: cjMara
Yes. This antibody has been featured in over a dozen journal articles, including 5 within the last year featuring the use of the (A-5) clone for detection of endogenous Wnt-5a in Western blotting applications. Product citations are available on our website.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by from Works nicely in IFWe trialed this antibody for IF and it works well on various cell lines. We have now bought the full size version.
Date published: 2020-03-04
Rated 5 out of 5 by from Good HRP signalWe use the unconjugated form and always had trouble with the signal. We tried this HRP conjugated form and it is very good. You should greatly reduce the concentration and/or time of blotting due to the HRP signal. Titrate the antibody for your application and you will get rid of the non specific bands.
Date published: 2018-06-07
Rated 4 out of 5 by from Works wellI tried the sample last month and it worked well!!
Date published: 2018-03-19
Rated 4 out of 5 by from Great!This monoclonal antibody gave robust and specific band in WB.
Date published: 2017-12-27
Rated 4 out of 5 by from Try it!This antibody works very well in WB. A strong and clean signal at the predicted M.W on the WB.
Date published: 2017-12-21
Rated 5 out of 5 by from positive bandWe study wn5a relations with tumor tissue.I use this antibody for first time, got the specific band of human kidney tumor extracts
Date published: 2017-06-25
Rated 5 out of 5 by from GreatGreat antibody for Western, strong band detected in human recombinant Wnt-5a.
Date published: 2017-02-07
Rated 5 out of 5 by from Good WB antibodyThis monoclonal antibody gave nice and strong band
Date published: 2017-01-16
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Wnt-5a 抗体 (A-5) is rated 4.7 out of 5 by 13.
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