WIP Antibody (E-9): sc-271114

WIP Antibody (E-9) is a mouse monoclonal IgG1 antibody that detects WIP in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). WIP, or WAS-interacting protein, plays a crucial role in the regulation of actin dynamics and is essential for proper immune cell function. WIP (E-9) antibody recognizes multiple domains that facilitate actin-binding and interaction with signaling proteins, which are vital for T cell and B cell activation. The interaction between WIP and the Wiskott-Aldrich syndrome protein (WASP) is particularly significant, as this binding is necessary for the activation of NF-AT/AP-1 transcription factors, which are critical for T cell responses. Mutations in WASP can disrupt this complex, leading to immunodeficiency, highlighting the importance of WIP in maintaining immune system integrity. The gene encoding human WIP is located on chromosome 2p23.3-q24.3, and WIP expression is associated with increased F-actin content and morphological changes in B cells, further underscoring its role in cytoskeletal organization and immune function. Anti-WIP antibody (E-9) serves as an invaluable tool for researchers studying the mechanisms of immune response and the pathogenesis of immunodeficiencies.

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WIP Antibody (E-9) References:

1. [Molecular pathogenesis of Wiskott-Aldrich syndrome].  |  Sasahara, Y. and Tsuchiya, S. 2005. Nihon Rinsho Meneki Gakkai Kaishi. 28: 140-7. PMID: 15997177
2. Downregulation of Wip-1 phosphatase expression in MCF-7 breast cancer cells enhances doxorubicin-induced apoptosis through p53-mediated transcriptional activation of Bax.  |  Kong, W., et al. 2009. Cancer Biol Ther. 8: 555-63. PMID: 19242108
3. WIP provides an essential link between Nck and N-WASP during Arp2/3-dependent actin polymerization.  |  Donnelly, SK., et al. 2013. Curr Biol. 23: 999-1006. PMID: 23707428
4. Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages.  |  Vijayakumar, V., et al. 2015. J Cell Sci. 128: 251-65. PMID: 25413351
5. Invadopodia proteins, cortactin, N-WASP and WIP differentially promote local invasiveness in ameloblastoma.  |  Siar, CH., et al. 2016. J Oral Pathol Med. 45: 591-8. PMID: 26752341
6. WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels.  |  Salvi, A. and Thanabalu, T. 2017. Biochem Biophys Res Commun. 482: 1353-1359. PMID: 27939884
7. WIP-YAP/TAZ as A New Pro-Oncogenic Pathway in Glioma.  |  Rivas, S., et al. 2018. Cancers (Basel). 10: PMID: 29890731
8. The Lack of WIP Binding to Actin Results in Impaired B Cell Migration and Altered Humoral Immune Responses.  |  Keppler, SJ., et al. 2018. Cell Rep. 24: 619-629. PMID: 30021160
9. WIPF2 promotes Shigella flexneri actin-based motility and cell-to-cell spread.  |  Michard, C., et al. 2019. Cell Microbiol. 21: e13098. PMID: 31414511
10. Spatiotemporal coordination of actin regulators generates invasive protrusions in cell-cell fusion.  |  Lu, Y., et al. 2024. Nat Cell Biol. 26: 1860-1877. PMID: 39487253

The preferred WIP antibody is WIP Antibody (C-1): sc-390099.