WDTC1 CRISPR/Cas9 KO Plasmid (h): sc-411657
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDTC1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDTC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDTC1 CRISPR/Cas9 KO Plasmid (h) | sc-411657 | 20 µg | $397.00 |
Overview
WDTC1 (also known as adipogenesis regulatory factor) encodes a WD repeat and TPR domain–containing protein implicated in the control of lipid storage and adipocyte differentiation programs. Reported functions link WDTC1 to ubiquitin-dependent regulatory mechanisms and transcriptional networks that influence cellular energy balance, with downstream effects on adipogenic gene expression and metabolic homeostasis. Altered WDTC1 activity has been associated with phenotypes relevant to obesity and broader metabolic dysregulation, making it useful for mechanistic studies of fat accumulation and nutrient-responsive signaling. In cell models, WDTC1 perturbation can help interrogate pathways governing lipid droplet biology, adipogenesis, and metabolic stress responses.
WDTC1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WDTC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WDTC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WDTC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDTC1 protein expression.
This CRISPR knockout system enables efficient generation of WDTC1-deficient cell models for investigation of WDTC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WDTC1 exon(s) critical for WDTC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WDTC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDTC1 CRISPR/Cas9 KO Plasmid (h) and WDTC1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WDTC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDTC1 HDR Plasmid (h) and WDTC1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WDTC1 homology arms to support homology-directed repair at defined WDTC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.