Date published: 2025-9-8
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VSV-G Antibody (F-6) - Western Blotting - Image 146314
VSV-G 抗体 (F-6): sc-365019. VSV-G 重组多融合标记蛋白的 Western 印迹分析.
VSV-G Antibody (F-6) - Western Blotting - Image 146314
VSV-G 抗体 (F-6): sc-365019. VSV-G 重组多融合标记蛋白的 Western 印迹分析.
VSV-G Antibody (F-6) - Western Blotting - Image 146315
VSV-G 抗体 (F-6): sc-365019. VSV-G 重组多融合标记蛋白的 Western 印迹分析.
VSV-G 抗体 (F-6): sc-365019. VSV-G 重组多融合标记蛋白的 Western 印迹分析.

VSV-G 抗体 (F-6): sc-365019

4.7(7)
寫評論提問

说明书
  • VSV-G 抗体 F-6 是小鼠单克隆 IgG2b κ，VSV-G抗体, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于Vesicular Stomatitis virus物种的VSV-G的C-terminus的氨基酸493-511之间
  • VSV-G 抗体 (F-6) 推荐用于WB, IP, IF 和 ELISA，检测VSV 来源的 VSV-G
  • 抗VSV-G抗体（F-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VSV-G (F-6): sc-365019的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是VSV-G Antibody (F-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与VSV-G Antibody (F-6) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

VSV抗体

VSV-G抗体（F-6）是一种IgG2bκ小鼠单克隆VSV-G抗体（也称为印第安纳囊泡病毒G抗体或VSVG抗体），能够通过WB、IP、IF和ELISA检测VSV来源的VSV-G蛋白。VSV-G抗体（F-6）既以非偶联型抗VSV-G抗体形式供应，也提供多种偶联型抗VSV-G抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。水疱性口炎病毒（VSV）是弹状病毒科、单负链病毒目的一种虫媒病毒。VSV可感染昆虫和哺乳动物。VSV的基因组是一条负义RNA链，编码五种主要蛋白：糖蛋白（G）、基质蛋白（M）、核蛋白（NC）、大蛋白（L）和磷蛋白。L蛋白和磷蛋白共同催化VSV的mRNA复制。在内吞作用后，G蛋白通过控制病毒与宿主细胞的附着以及病毒包膜与内体膜的融合，促进VSV进入细胞。G蛋白从内质网（ER）到质膜（PM）的转运对温度敏感。因此，VSV常被用于研究实验室中研究弹状病毒科病毒的特性以及病毒进化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VSV-G 抗体 (F-6) 参考文献:

  1. 水泡性口炎病毒 G 伪型逆转录载体在实验性脑癌中介导了有效的体内自杀式基因递送。  |  Galipeau, J., et al. 1999. Cancer Res. 59: 2384-94. PMID: 10344748
  2. 疱疹性口炎病毒糖蛋白质粒在肿瘤中的致熔活性可增强 IL-12 基因疗法。  |  Eslahi, NK., et al. 2001. Cancer Gene Ther. 8: 55-62. PMID: 11219494
  3. 过渡性ER是tsO45 VSV糖蛋白分泌过程中质量控制的边界。  |  Mezzacasa, A. and Helenius, A. 2002. Traffic. 3: 833-49. PMID: 12383349
  4. 水泡性口炎病毒糖蛋白的跨膜结构域足以锚定由重组疫苗病毒表达的 HIV-1 包膜 gp120。  |  Jin, Y. and Wright, SE. 2003. Int J Mol Med. 12: 11-6. PMID: 12792802
  5. 表达水泡性口炎病毒糖蛋白的重组淋巴细胞性脉管炎病毒。  |  Pinschewer, DD., et al. 2003. Proc Natl Acad Sci U S A. 100: 7895-900. PMID: 12808132
  6. dl-VSVG-LacZ 是一种水泡性口炎病毒糖蛋白表位结合的腺病毒，可显著提高基因转导效率。  |  Yun, CO., et al. 2003. Hum Gene Ther. 14: 1643-52. PMID: 14633406
  7. 表达博尔纳病病毒糖蛋白的重组水泡性口炎病毒的产生和特征。  |  Perez, M., et al. 2007. J Virol. 81: 5527-36. PMID: 17376911
  8. 水泡性口炎病毒-G（VSV-G）共轭物的制备及其在基因转移中的应用。  |  Miyanohara, A. 2012. Cold Spring Harb Protoc. 2012: 453-6. PMID: 22474657
  9. 包装基于 HIV 或 FIV 的激励介导表达构建体和转导 VSV-G 伪型病毒颗粒。  |  Mendenhall, A., et al. 2012. J Vis Exp. e3171. PMID: 22508377
  10. 狒狒包膜伪型 LV 在将基因转移到早期细胞因子刺激的和静止的造血干细胞方面优于 VSV-G-LV。  |  Girard-Gagnepain, A., et al. 2014. Blood. 124: 1221-31. PMID: 24951430
  11. VSV G 蛋白的胞内转运发生在缺乏核包膜的细胞中。  |  Hanover, JA. 1988. Biochem Biophys Res Commun. 152: 469-76. PMID: 2833898
  12. 在极化和非极化细胞中，水泡性口炎病毒 G 蛋白向细胞表面的迁移是由信号介导的。  |  Müsch, A., et al. 1996. J Cell Biol. 133: 543-58. PMID: 8636230

订购信息

产品名称产品编号规格价格数量收藏夹

VSV-G 抗体 (F-6)

sc-365019
200 µg/ml
$316.00

VSV-G (F-6): m-IgG2b BP-HRP 套装

sc-548715
200 µg Ab; 10 µg BP
$354.00

VSV-G 抗体 (F-6) AC

sc-365019 AC
500 µg/ml, 25% agarose
$416.00

VSV-G 抗体 (F-6) HRP

sc-365019 HRP
200 µg/ml
$316.00

VSV-G 抗体 (F-6) FITC

sc-365019 FITC
200 µg/ml
$330.00

VSV-G 抗体 (F-6) PE

sc-365019 PE
200 µg/ml
$343.00

VSV-G 抗体 (F-6) Alexa Fluor® 488

sc-365019 AF488
200 µg/ml
$357.00

VSV-G 抗体 (F-6) Alexa Fluor® 546

sc-365019 AF546
200 µg/ml
$357.00

VSV-G 抗体 (F-6) Alexa Fluor® 594

sc-365019 AF594
200 µg/ml
$357.00

VSV-G 抗体 (F-6) Alexa Fluor® 647

sc-365019 AF647
200 µg/ml
$357.00

VSV-G 抗体 (F-6) Alexa Fluor® 680

sc-365019 AF680
200 µg/ml
$357.00

VSV-G 抗体 (F-6) Alexa Fluor® 790

sc-365019 AF790
200 µg/ml
$357.00

VSV-G (F-6) 中和勝肽

sc-365019 P
100 µg/0.5 ml
$68.00

Hello. I wonder whether or not WB should be done under reductive condition with this antibody.

Asked by: RAEXO
Thank you very much for your question.Please refer to the protocol for details: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Jiajun Z
Date published: 2025-08-13

Hello, I wonder if this antibody can be used in flow cytometry against intact virus and intact cells. As it recognizes the intravirion region of the VSV (493-511), can the antibody interact with VSV in the context of intact virions/cells?

Asked by: Solrac
Thank you for your question. VSV-G Antibody (F-6) is only recommended for detection of VSV-G of VSV origin by WB, IP, IF and ELISA. We do not have any internal data supporting flow cytometry of intact cells or virions.
Answered by: Tech Support Europe
Date published: 2021-07-23

Hello, writing here from the University of MN Dept of Infectious Disease. Does this anti-VSV-G ab neutralize VSV?

Asked by: Eva97
Thanks for your question.Our product is not recommended in vivo.Please contact our Technical Service Department for further questions.Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support 9
Date published: 2020-06-12

Hello! Is the VSV-G Antibody (F-6): sc-365019 raised against actual VSV-G virus, or just against the peptide? Thanks, Fiona

Asked by: FionaH
Thank you for your question. This antibody was raised against a peptide with a sequence of VSV-G origin. It was not made against the actual VSV-G virus.
Answered by: Technical Support
Date published: 2019-01-26

the storage manual on product told me, it could be storaged in 4℃, and i want to know about if i store it on room temp. does it make enormous difference?

Asked by: Jinwoo
Thank you for your question. The antibodies should always be stored at 4˚C for long term storage, but generally they are OK at room temperature for a day or two. We do not recommend storing them at room temperature long term. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2018-12-30

What is the recommended secondary to use with this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13

How should the blocking peptide sc-365019 P be used?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended to be used as a negative control by preadsorbing the VSV-G antibody sc-365019 with it prior to application. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 3
Date published: 2017-01-13
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Rated 5 out of 5 by from 良いですSf9細胞へのVSV-Gバキュロウイルスの感染確認のためにサンプルを提供して頂きました。固定細胞を用いて蛍光抗体法で検出しましたが（二次抗体：Alexa488-抗マウス IgG、1/500）、1/500-1/1000の希釈率できれいに検出することが出来ました。
Date published: 2021-11-05
Rated 4 out of 5 by from Good antibody for FACSI used the Alexa-647 conjugated antibody on FACS and it works really nice! Dilution 1:200
Date published: 2021-07-01
Rated 5 out of 5 by from Great antibodyPositive highly specific band observed while using this antibody to detect VSV-G protein levels in VSV infected cells by WB (at the dilution of 1:500).
Date published: 2018-03-27
Rated 4 out of 5 by from A good antibody!I Used this one to do WB and detect the target protein in medium. It looks like good and background is quite clean.
Date published: 2017-04-13
Rated 5 out of 5 by from Stong signal in WBThis VSV-G Antibody shows strong signal in western blotting of human samples.
Date published: 2017-01-11
Rated 5 out of 5 by from Worked well for my western blotI received a sample of this antibody and it worked well for my western blot - bound specifically and yielded a strong signal.
Date published: 2016-10-27
Rated 5 out of 5 by from Produced positive Western Blot data of VSVProduced positive Western Blot data of VSV-G recombinant multi-fusion tagged protein. -SCBT QC
Date published: 2013-09-16
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VSV-G 抗体 (F-6) is rated 4.7 out of 5 by 7.
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