Date published: 2025-9-6

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VPS4A 抗体 (A-11): sc-393428

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说明书
  • VPS4A抗体(A-11)是小鼠单克隆IgG2b κ, 在11篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的VPS4A的C-terminus附近的氨基酸349-370之间
  • 抗-VPS4A 抗体 (A-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 VPS4A
  • 抗VPS4A抗体(A-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VPS4A (A-11): sc-393428免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是VPS4A Antibody (A-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与VPS4A Antibody (A-11) 打包提供(请参阅下面的订购信息)。

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関連項目

VPS4A抗体(A-11)是一种IgG2b κ小鼠单克隆VPS4A抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的VPS4A。VPS4A抗体(A-11)既可作为非共轭抗VPS4A抗体形式提供,也可作为多种共轭形式的抗VPS4A抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。E类液泡蛋白分选(vps)蛋白对于酵母内吞途径中受体的适当分选至关重要。酵母Vps4p是AAA蛋白家族(与多种细胞活动相关的ATP酶)的成员,并在将蛋白质从液泡前内体区室中运输出来方面发挥重要作用。在人类中,已知存在两种非等位直系同源蛋白VPS4A和VPS4B,它们可以相互形成异聚体复合物。VPS4A和VPS4B都是E类VPS蛋白,并涉及细胞内蛋白质运输,与酵母中的Vps4p相似。HIV-1利用细胞机制从受感染的细胞中出芽,并且需要两种人类VPS4蛋白和tsg 101(肿瘤易感基因101)来进行此出芽过程。VPS4蛋白的显性负突变体抑制液泡蛋白分选,并且还阻止HIV-1和MLV出芽。因此,逆转录病毒通常在出芽过程中使用VPS途径来形成多泡体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VPS4A 抗体 (A-11) 参考文献:

  1. TSG101/哺乳动物 VPS23 和哺乳动物 VPS28 直接相互作用,并被招募到 VPS4 诱导的内体。  |  Bishop, N. and Woodman, P. 2001. J Biol Chem. 276: 11735-42. PMID: 11134028
  2. CHMP1 是一个新定义的囊泡贩运蛋白家族的成员。  |  Howard, TL., et al. 2001. J Cell Sci. 114: 2395-404. PMID: 11559748
  3. 哺乳动物细胞表达两种 VPS4 蛋白,它们都参与细胞内蛋白质的运输。  |  Scheuring, S., et al. 2001. J Mol Biol. 312: 469-80. PMID: 11563910
  4. Tsg101和液泡蛋白分拣途径对HIV-1的萌发至关重要。  |  Garrus, JE., et al. 2001. Cell. 107: 55-65. PMID: 11595185
  5. 抵抗是徒劳的:HIV-1 对细胞机制的同化。  |  Perez, OD. and Nolan, GP. 2001. Immunity. 15: 687-90. PMID: 11728331
  6. 四种哺乳动物 VPS4 基因的序列和表达比较分析。  |  Beyer, A., et al. 2003. Gene. 305: 47-59. PMID: 12594041
  7. VPS4A-外泌体途径在 DNA 结合型抗癌药物的内在排出途径中的作用。  |  Chen, VY., et al. 2006. Pharm Res. 23: 1687-95. PMID: 16841193
  8. 泛素耗竭和显性阴性 VPS4 可抑制横纹肌病毒的出芽,但不影响阿尔法病毒的出芽。  |  Taylor, GM., et al. 2007. J Virol. 81: 13631-9. PMID: 17913808
  9. VPS4 ATPases 识别 ESCRT-III。  |  Stuchell-Brereton, MD., et al. 2007. Nature. 449: 740-4. PMID: 17928862
  10. Vps4a调节自噬通量以预防肥厚型心肌病  |  Huang, X., et al. 2023. Int J Mol Sci. 24: PMID: 37445978
  11. 阿洛哌啶通过与 VPS4A 相互作用抑制 NSCLC 中自噬体与溶酶体的融合来抑制癌症进展  |  Guo, W., et al. 2024. Adv Sci (Weinh). 11: e2308307. PMID: 39166458
  12. VPS4A 是小鼠和人类噬脂的选择性受体。  |  Das, D., et al. 2024. Mol Cell. 84: 4436-4453.e8. PMID: 39520981

订购信息

产品名称产品编号规格价格数量收藏夹

VPS4A 抗体 (A-11)

sc-393428
200 µg/ml
$316.00

VPS4A (A-11): m-IgG Fc BP-HRP 套装

sc-530454
200 µg Ab; 10 µg BP
$354.00

VPS4A (A-11): m-IgGκ BP-HRP 套装

sc-523943
200 µg Ab, 40 µg BP
$354.00

VPS4A (A-11): m-IgG2b BP-HRP 套装

sc-550190
200 µg Ab; 10 µg BP
$354.00

VPS4A 抗体 (A-11) AC

sc-393428 AC
500 µg/ml, 25% agarose
$416.00

VPS4A 抗体 (A-11) HRP

sc-393428 HRP
200 µg/ml
$316.00

VPS4A 抗体 (A-11) FITC

sc-393428 FITC
200 µg/ml
$330.00

VPS4A 抗体 (A-11) PE

sc-393428 PE
200 µg/ml
$343.00

VPS4A 抗体 (A-11) Alexa Fluor® 488

sc-393428 AF488
200 µg/ml
$357.00

VPS4A 抗体 (A-11) Alexa Fluor® 546

sc-393428 AF546
200 µg/ml
$357.00

VPS4A 抗体 (A-11) Alexa Fluor® 594

sc-393428 AF594
200 µg/ml
$357.00

VPS4A 抗体 (A-11) Alexa Fluor® 647

sc-393428 AF647
200 µg/ml
$357.00

VPS4A 抗体 (A-11) Alexa Fluor® 680

sc-393428 AF680
200 µg/ml
$357.00

VPS4A 抗体 (A-11) Alexa Fluor® 790

sc-393428 AF790
200 µg/ml
$357.00

VPS4A (A-11) 中和勝肽

sc-393428 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-393428 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Nice WBNice Western Blot data of VPS4A expression in HeLa whole cell lysate (25 μg protein lysate; ab dilution 1:1000; incubation at 4°C overnight)
Date published: 2021-07-23
Rated 3 out of 5 by from a lot of unspecific bandsI've tested this antibody in parallel with a polyclonal antibody from another company in human fibroblasts. A lot of unspecific signals were observed for the VPS4A A-11 antibody. If you know which is the specific band, its fine. In IF it only shows a cytosolic signal.
Date published: 2018-01-23
Rated 5 out of 5 by from Very good antibodyI used this antibody. It works very well and specific. There is no unspecific band.
Date published: 2017-09-06
Rated 5 out of 5 by from Excellent choice for VPS4A detection!This VPS4A mono is a great choice, especially if you are looking for a solid antibody for Western blot. Great choice!
Date published: 2017-02-03
Rated 5 out of 5 by from Nice Western Blot data of VPS4 expressionNice Western Blot data of VPS4 expression in Hep G2 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Good Western Blot data of VPS4A expression in nonGood Western Blot data of VPS4A expression in non-transfected 293T, human VPS4A transfected 293T and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-04-16
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VPS4A 抗体 (A-11) is rated 4.5 out of 5 by 6.
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