Date published: 2025-9-6
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VDUP1 Antibody (D-2) - Western Blotting - Image 374742
VDUP1 抗体 (D-2): sc-271237. HL-60 (A), K-562 (B), HEL 92. 1. 7 (C) 和 THP-1 (D) 全细胞裂解液中 VDUP1 表达的 Western 印迹分析.
VDUP1 抗体 (D-2): sc-271237. HL-60 (A), K-562 (B), HEL 92. 1. 7 (C) 和 THP-1 (D) 全细胞裂解液中 VDUP1 表达的 Western 印迹分析.

VDUP1 抗体 (D-2): sc-271237

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寫評論提問

说明书
  • VDUP1 抗体 D-2 是小鼠单克隆 IgG1 κ，VDUP1抗体, 在14篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的VDUP1的C-terminus的氨基酸342-370之间
  • VDUP1 抗体 (D-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 VDUP1
  • 抗VDUP1抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VDUP1 (D-2): sc-271237的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是VDUP1 Antibody (D-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与VDUP1 Antibody (D-2) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

VDUP1抗体（D-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的VDUP1。VDUP1 (D-2) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。VDUP1，也称为硫氧还蛋白相互作用蛋白，在细胞应激反应中起着至关重要的作用，在氧化应激、紫外线辐射和热休克反应中，1,25(OH)2D3会上调VDUP1的表达。VDUP1是硫氧还蛋白的天然拮抗剂，通过诱导细胞周期在G0/G1期停滞来抑制肿瘤，这对于防止细胞无序增殖至关重要。此外，VDUP1对于CD122的表达和自然杀伤（NK）细胞的成熟至关重要，尽管VDUP1对T和B细胞发育的影响微乎其微。编码人类VDUP1的基因位于1q21染色体上，VDUP1在各种组织中普遍表达，主要定位于细胞质中。鉴于VDUP1在免疫调节中的重要作用以及作为黑色素瘤治疗靶标的潜力，VDUP1仍然是癌症生物学和免疫学研究的重要焦点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

VDUP1 抗体 (D-2)

sc-271237
200 µg/ml
$316.00

VDUP1 (D-2): m-IgGκ BP-HRP 套装

sc-521887
200 µg Ab, 40 µg BP
$354.00

VDUP1 抗体 (D-2) AC

sc-271237 AC
500 µg/ml, 25% agarose
$416.00

VDUP1 抗体 (D-2) HRP

sc-271237 HRP
200 µg/ml
$316.00

VDUP1 抗体 (D-2) FITC

sc-271237 FITC
200 µg/ml
$330.00

VDUP1 抗体 (D-2) PE

sc-271237 PE
200 µg/ml
$343.00

VDUP1 抗体 (D-2) Alexa Fluor® 488

sc-271237 AF488
200 µg/ml
$357.00

VDUP1 抗体 (D-2) Alexa Fluor® 546

sc-271237 AF546
200 µg/ml
$357.00

VDUP1 抗体 (D-2) Alexa Fluor® 594

sc-271237 AF594
200 µg/ml
$357.00

VDUP1 抗体 (D-2) Alexa Fluor® 647

sc-271237 AF647
200 µg/ml
$357.00

VDUP1 抗体 (D-2) Alexa Fluor® 680

sc-271237 AF680
200 µg/ml
$357.00

VDUP1 抗体 (D-2) Alexa Fluor® 790

sc-271237 AF790
200 µg/ml
$357.00

VDUP1 (D-2) 中和勝肽

sc-271237 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271237 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Non-specificThis antibody doesnt work properly in order to detect VDUP1 by western blot in whole cell lysates (BT474 cell line). It is non-specific.
Date published: 2018-07-24
Rated 3 out of 5 by from I don`t know which band is the right band1:250 diluted for wb of rat protein of 50 ug.The band is clear,but there are two main bands ,one is above 55kd,the other is below 40KD ,both of them are strong,I don`t know which one is the right one
Date published: 2017-07-29
Rated 5 out of 5 by from Great with IP and WB for skeletal muscle tissueOrdered a sample of this product to use for both IP and WB on skeletal muscle tissue (100ug for IP) the band that appeared was quite robust for both the straight tissue homogenate and the IP'd sample
Date published: 2017-03-23
Rated 4 out of 5 by from Works fineGot the antibody as a free sample (20ul) which was good for one blot. Worked good as i could clearly see the band of my interset but had couple of non-specific bands as well near the main band.
Date published: 2017-03-08
Rated 5 out of 5 by from Specific and robust in WBGreat choice if you are looking for a solid antibody for VDUP1, nice strong western results!
Date published: 2017-02-02
Rated 5 out of 5 by from Used this antibody in WB and IP in mouseUsed this antibody in WB and IP in mouse lung tissues- got correct band at 1:500 dilution.
Date published: 2015-10-28
Rated 5 out of 5 by from Clean bands in western blot using HLClean bands in western blot using HL-60 and M1 whole cell lysates and human kidney tissue extract. -SCBT QC
Date published: 2013-12-03
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VDUP1抗体 (D-2) is rated 4.0 out of 5 by 7.
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