Date published: 2025-9-7
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Vitamin D Receptor/VDR Antibody (D-6) - Western Blotting - Image 362199
VDR 抗体 (D-6): sc-13133. HeLa (A), MCF7 (B) 和 C3H/10T1/2 (C) 全细胞裂解液中 VDR 表达的 Western 印迹分析.
VDR 抗体 (D-6): sc-13133. HeLa (A), MCF7 (B) 和 C3H/10T1/2 (C) 全细胞裂解液中 VDR 表达的 Western 印迹分析.

Vitamin D Receptor/VDR 抗体 (D-6): sc-13133

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说明书
  • Vitamin D Receptor/VDR 抗体 D-6 是小鼠单克隆 IgG2a κ，Vitamin D Receptor/VDR抗体, 在437篇文献中引用，规格为200 µg/ml
  • 免疫human物种的VDR的氨基酸344-424
  • Vitamin D Receptor/VDR 抗体 (D-6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 VDR
  • 抗Vitamin D Receptor/VDR抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13133 X, 200 µg/0.1 ml)
  • 关于如何获取Vitamin D Receptor/VDR (D-6): sc-13133的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Vitamin D Receptor/VDR 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Vitamin D Receptor/VDR 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

VDR抗体（D-6）是一种IgG2a κ小鼠单克隆VDR抗体（也称为维生素D受体抗体或NR1I1抗体），可通过WB、IP、IF和IHC（P）检测来自小鼠、大鼠和人类的VDR蛋白。VDR抗体（D-6）既可以是非偶联的抗VDR抗体形式，也可以是多种偶联形式的抗VDR抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。维生素D的活性代谢物以发育特异性的方式调节多种基因的表达。这种环类固醇激素可以上调或下调参与多种反应（如增殖、分化和钙稳态）的基因表达。1,25-（OH）2-维生素D3通过与维生素D受体（VDR）的相互作用来发挥其作用，VDR是被激素激活的核受体超家族的成员。在其配体结合状态下，VDR与9-cis维甲酸受体RXR形成异构二聚体，并通过结合称为激素反应元件（HREs）的特定DNA序列来影响基因表达。除了调节上述细胞反应，1,25-(OH)2-维生素D3在骨肉瘤，黑色素瘤，结肠癌和乳腺癌细胞中表现出抗增殖特性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Vitamin D Receptor/VDR 抗体 (D-6) 参考文献:

  1. 维生素 D 介导的基因表达。  |  Lowe, KE., et al. 1992. Crit Rev Eukaryot Gene Expr. 2: 65-109. PMID: 1543898
  2. 维生素 D 与前列腺癌  |  Feldman, D., et al. 1995. Adv Exp Med Biol. 375: 53-63. PMID: 7645428
  3. 1,25-二羟维生素 D3 的基因组作用。  |  Whitfield, GK., et al. 1995. J Nutr. 125: 1690S-1694S. PMID: 7782928
  4. 乳腺癌细胞中的维生素 D 受体  |  Buras, RR., et al. 1994. Breast Cancer Res Treat. 31: 191-202. PMID: 7881099
  5. 维生素 D, 其代谢物和类似物在骨质疏松症治疗中的作用。  |  Bikle, DD. 1994. Rheum Dis Clin North Am. 20: 759-75. PMID: 7984788
  6. 维生素 D 诱导分化的信号通路：对治疗增殖性和肿瘤性疾病的意义。  |  Studzinski, GP., et al. 1993. Crit Rev Eukaryot Gene Expr. 3: 279-312. PMID: 8286848

订购信息

产品名称产品编号规格价格数量收藏夹

Vitamin D Receptor/VDR 抗体 (D-6)

sc-13133
200 µg/ml
$316.00

Vitamin D Receptor/VDR (D-6): m-IgG Fc BP-HRP 套装

sc-525466
200 µg Ab; 10 µg BP
$354.00

Vitamin D Receptor/VDR (D-6): m-IgG2a BP-HRP 套装

sc-546272
200 µg Ab; 10 µg BP
$354.00

Vitamin D Receptor/VDR 抗体 (D-6) X

sc-13133 X
200 µg/0.1 ml
$316.00

Vitamin D Receptor/VDR 抗体 (D-6) AC

sc-13133 AC
500 µg/ml, 25% agarose
$416.00

Vitamin D Receptor/VDR 抗体 (D-6) HRP

sc-13133 HRP
200 µg/ml
$316.00

Vitamin D Receptor/VDR 抗体 (D-6) FITC

sc-13133 FITC
200 µg/ml
$330.00

Vitamin D Receptor/VDR 抗体 (D-6) PE

sc-13133 PE
200 µg/ml
$343.00

Vitamin D Receptor/VDR 抗体 (D-6) Alexa Fluor® 488

sc-13133 AF488
200 µg/ml
$357.00

Vitamin D Receptor/VDR 抗体 (D-6) Alexa Fluor® 546

sc-13133 AF546
200 µg/ml
$357.00

Vitamin D Receptor/VDR 抗体 (D-6) Alexa Fluor® 594

sc-13133 AF594
200 µg/ml
$357.00

Vitamin D Receptor/VDR 抗体 (D-6) Alexa Fluor® 647

sc-13133 AF647
200 µg/ml
$357.00

Vitamin D Receptor/VDR 抗体 (D-6) Alexa Fluor® 680

sc-13133 AF680
200 µg/ml
$357.00

Vitamin D Receptor/VDR 抗体 (D-6) Alexa Fluor® 790

sc-13133 AF790
200 µg/ml
$357.00

Vitamin D Receptor/VDR 抗体 (D-6) B

sc-13133 B
200 µg/ml
$320.00

Can we use the PE conjugated antibody for Western? Is there an antibody that works for both Western blot and flow cytometry (not image based western).

Asked by: KRMBCM
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-13

This antibody can be used for immunocytochemistry?

Asked by: Anonymous
Thank you for your question. Vitamin D Receptor/VDR Antibody (D-6): sc-13133 is recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and IHC(P), so it is suitable for immunocytochemistry.
Answered by: Tech Support Europe
Date published: 2022-11-16

Wondering how the Alexa Fluor® 680 or Alexa Fluor® 790 conjugated antibody works for flow cytometry? Would be good to get a list of publications where it is specifically used for flow as the list of publications is lengthy...

Asked by: Halip5
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-18

Hi there, since the VDR has different isoforms, I am wondering the amino acids sequence for VDR this antibody corresponding. Thanks!

Asked by: Fay Liu
Thank you for your question. VDR Antibody (D-6): sc-13133 was raised against amino acids 344-424 of VDR isoform 1 of human origin. This is the corresponding amino acid sequence: PGVQDAA LIEAIQDRLS NTLQTYIRCR HPPPGSHLLY AKMIQKLADL RSLNEEHSKQ YRCLSFQPEC SMKLTPLVLE VFGN Please do not hesitate to contact our technical service directly if you have additional questions.
Answered by: Tech Support Europe
Date published: 2019-06-05

does D6 vitamin d receptor antibody cross react with monkey (macaques) for immunohistochemistry

Asked by: schristak
Thank you for your question. This antibody has not been tested on monkey samples in house, so it is not technically recommended or warrantied for that use, however, we do have publications in monkey (rhesus) samples.
Answered by: Tech Service
Date published: 2019-04-09

Hello, I have been working with another VDR and I am not certain I am fully appreciating that VDR. Hence I am interested in the "Contact us to receive a FREE 10 µg sample of VDR (D-6): sc-13133." offer of getting 10 ug for free. Is it possible?

Asked by: hanna abrahamsson
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Can Santa Cruz provide VDR IHC images (performed using D-6 sc-13133 ab) with staining intensity (score) of 1 to 4 and % positive stained cells (score) with 1-4 (preferably in FFPE colorectal tumor tissue samples) ?

Asked by: Sanbc
Thank you for the question. Please note that this product is sold for research or laboratory use only and it must not been used for medical diagnostics. All available IHC(P) images for this product will be published upon availability on our product website. We currently show staining of human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells, human skin tissue showing nuclear and cytoplasmic staining of keratinocytes, fibroblasts, Langerhans cells and melanocytes and human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Answered by: Technical Support Europe
Date published: 2018-01-10

I got a double band when I used this antibody, any idea what the other band could be? Thanks

Asked by: Reham
Thank you very much for your inquiry. Please understand that we do not interpret customer data, since we do not have a detailed knowledge about your experiment and how the data was produced. Therefore our interpretation of the data could lead to a wrong direction without knowing all the relevant details. However, Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Common factors include: 1. Post-translational modification - e.g. phosphorylation, glycosylation etc, which increases the size of the protein. 2. Post-translation cleavage - e.g. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. pro-caspases. 3. Splice variants - alternative splicing may create different sized proteins produced from the same gene. 4. Relative charge - the composition of amino acids (charged vs non-charged). 5. Multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
Answered by: Technical Support Europe
Date published: 2021-05-09
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Rated 5 out of 5 by from 组化效果不错一个月前申请了试用装，用来做石蜡切片的组化效果确实可以的，考虑买个正装。如果正装价格还能有折扣就好了
Date published: 2024-04-19
Rated 1 out of 5 by from Cambio de loteEste anticuerpo funcionaba muy bien, pero el nuevo lote #A0621 no funciona ni para western-blot, ni para inmunoprecipitación ni para inmunofluorescencia
Date published: 2021-10-29
Rated 5 out of 5 by from Worked well in human and mouse muscle tissue.I used this antibody for IHC and got good results following published protocols. I used the unconjugated primary antibody and had to amplify for the human samples, but the mouse samples worked well going straight into secodary.
Date published: 2021-01-25
Rated 3 out of 5 by from low dilutionI needed to use a low dilution to make it work (1:50)
Date published: 2020-09-22
Rated 5 out of 5 by from Buen anticuerpoTiene una alta especificidad, en wblot da una muy buena señal y se puede reutilizar varias veces.
Date published: 2020-09-01
Rated 5 out of 5 by from very good antibodyThis antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Date published: 2019-01-02
Rated 5 out of 5 by from Best AntibodyWe have tried this antibody and published. Instruction and protocol was well described. I strongly recommend this antibody to other researcher.
Date published: 2018-08-24
Rated 5 out of 5 by from EXCELLENT SENSITIVITYTO SEE VDR ON PROTEIN LEVEL IS VERY TOUGH, BUT USING THE HYPEROSMOLAR BUFFER LYSIS AND THIS VDR ANTIBODY. I GOT THE BAND OF VDR. THIS ANTIBODY IS BETTER THAN ABCAM. THANK YOU
Date published: 2018-04-12
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Vitamin D Receptor/VDR 抗体 (D-6) is rated 4.5 out of 5 by 32.
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