Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VAP-1 CRISPR/Cas9 KO Plasmid (h): sc-401777
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VAP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VAP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VAP-1 Antibody (A-8): sc-166713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VAP-1 CRISPR/Cas9 KO Plasmid (h) | sc-401777 | 20 µg | $397.00 |
Overview
AOC3 encodes vascular adhesion protein-1 (VAP-1), an endothelial and smooth muscle cell surface molecule that functions as a leukocyte adhesion receptor and as a copper-dependent semicarbazide-sensitive amine oxidase (SSAO). Through oxidative deamination of primary amines, VAP-1 generates aldehydes, ammonia, and hydrogen peroxide, linking vascular redox biology to regulation of immune cell trafficking across the endothelium. VAP-1 activity intersects with inflammatory signaling, cell–cell adhesion dynamics, and extracellular matrix remodeling within the vascular microenvironment. Dysregulated AOC3/VAP-1 expression or enzymatic activity has been associated with chronic inflammatory states and vascular pathobiology, supporting its use as a mechanistic node in studies of endothelial activation and leukocyte extravasation.
VAP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AOC3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AOC3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AOC3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VAP-1 protein expression.
This CRISPR knockout system enables efficient generation of AOC3-deficient cell models for investigation of VAP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AOC3 exon(s) critical for VAP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AOC3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VAP-1 CRISPR/Cas9 KO Plasmid (h) and VAP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AOC3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VAP-1 HDR Plasmid (h) and VAP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AOC3 homology arms to support homology-directed repair at defined AOC3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.