Date published: 2025-9-6

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USP9X/Y 抗体 (E-12): sc-365353

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说明书
  • USP9X/Y 抗体 E-12 是小鼠单克隆 IgG2a κ,USP9X/Y抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列2248-2547位于human物种的USP9X/Y的C-terminus
  • USP9X/Y 抗体 (E-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测 USP9X of mouse, rat and human orgin, and USP9Y of human origin
  • 抗USP9X/Y抗体(E-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取USP9X/Y (E-12): sc-365353免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是USP9X/Y Antibody (E-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与USP9X/Y Antibody (E-12) 打包提供(请参阅下面的订购信息)。

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関連項目

USP9X/Y抗体(E-12)是一种IgG2aκ小鼠单克隆USP9X/Y抗体(也称为USP9X/USP9Y抗体),它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的USP9X/Y蛋白。USP9X/Y抗体(E-12)既可作为非偶联的抗USP9X/Y抗体形式存在,也可作为多种偶联形式的抗USP9X/Y抗体存在,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。泛素(Ub)途径涉及三个连续的酶促步骤,这些步骤促进Ub和Ub样分子与特定蛋白质底物的结合。通过使用可以添加或去除泛素的多种酶,Ub途径控制许多细胞内过程,如信号转导、转录激活和细胞周期进程。USP9X(泛素特异性肽酶9,X连锁),也称为FAF或DFFRX,是肽酶C19家族中由2547个氨基酸组成的泛素蛋白酶成员。USP9X在胎儿和成人组织中普遍表达,参与泛素前体和泛素化蛋白质的加工处理,从而防止降解并调节蛋白质周转。USP9Y(泛素特异性肽酶9,Y连锁)是肽酶C19家族的另一个成员,是一种由2555个氨基酸组成的蛋白质,广泛表达,与USP9X一样,在泛素前体和泛素化蛋白质的加工处理中起重要作用。编码USP9X的基因缺陷与特纳综合征有关,这是一种卵母细胞无法增殖和发育的病症,而编码USPPY的基因缺陷与非梗阻性无精子症和不孕症有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP9X/Y 抗体 (E-12) 参考文献:

  1. Usp9y(Y 上的泛素特异性蛋白酶 9 基因)与一个功能性启动子有关,并编码一个与 Usp9x 同源的完整开放阅读框,该阅读框受到选择性限制。  |  Hall, NM., et al. 2003. Mamm Genome. 14: 437-47. PMID: 12925892
  2. 双皮质素与泛素蛋白酶 DFFRX 相互作用,后者与神经元过程中的微管缔合。  |  Friocourt, G., et al. 2005. Mol Cell Neurosci. 28: 153-64. PMID: 15607950
  3. 参与突触发育的泛素特异性蛋白酶 Usp9x 在小鼠海马中的空间和时间特异性表达  |  Xu, J., et al. 2005. J Neurosci Res. 80: 47-55. PMID: 15723417
  4. 小鼠大脑中 Usp9x 蛋白表达和 DNA 甲基化与年龄有关的变化  |  Xu, J. 2005. Brain Res Mol Brain Res. 140: 17-24. PMID: 16023255
  5. 14-3-3 与 LKB1 合作调节 QSK 和 SIK 的活性和定位。  |  Al-Hakim, AK., et al. 2005. J Cell Sci. 118: 5661-73. PMID: 16306228
  6. 泛素连接酶 itch 在体内和体外都会被自动泛素化,但通过与去泛素化酶 FAM/USP9X 相互作用而免于降解。  |  Mouchantaf, R., et al. 2006. J Biol Chem. 281: 38738-47. PMID: 17038327
  7. USP9X 和非典型 Lys(29)/Lys(33)-linked 多泛素链对 AMPK 相关激酶的控制。  |  Al-Hakim, AK., et al. 2008. Biochem J. 411: 249-60. PMID: 18254724
  8. USP9X 去泛素化 ALDH1A3 并维持胶质母细胞瘤干细胞的间质特性。  |  Chen, Z., et al. 2019. J Clin Invest. 129: 2043-2055. PMID: 30958800
  9. USP9X 介导的 KDM4C 泛素化通过表观遗传诱导 TGF-β2 转录促进肺癌放射抗性。  |  Jie, X., et al. 2021. Cell Death Differ. 28: 2095-2111. PMID: 33558705
  10. 巨噬细胞中 USP9X 的破坏会促进泡沫细胞的形成和动脉粥样硬化。  |  Wang, B., et al. 2022. J Clin Invest. 132: PMID: 35389885
  11. 细胞功能、分子信号和治疗应用:去泛素化酶 USP9X 作为癌症治疗药物靶点的转化潜力。  |  Gao, H., et al. 2024. Biochim Biophys Acta Rev Cancer. 1879: 189099. PMID: 38582329
  12. 人类 DFFRY 基因的编码序列特征和精细图谱,以及 Dffry 基因的比较表达分析和与小鼠 Y 染色体 Sxrb 区间的图谱。  |  Brown, GM., et al. 1998. Hum Mol Genet. 7: 97-107. PMID: 9384609

订购信息

产品名称产品编号规格价格数量收藏夹

USP9X/Y 抗体 (E-12)

sc-365353
200 µg/ml
$316.00

USP9X/Y (E-12): m-IgG Fc BP-HRP 套装

sc-529356
200 µg Ab; 10 µg BP
$354.00

USP9X/Y (E-12): m-IgGκ BP-HRP 套装

sc-522217
200 µg Ab, 40 µg BP
$354.00

USP9X/Y (E-12): m-IgG2a BP-HRP 套装

sc-547303
200 µg Ab; 10 µg BP
$354.00

USP9X/Y 抗体 (E-12) AC

sc-365353 AC
500 µg/ml, 25% agarose
$416.00

USP9X/Y 抗体 (E-12) HRP

sc-365353 HRP
200 µg/ml
$316.00

USP9X/Y 抗体 (E-12) FITC

sc-365353 FITC
200 µg/ml
$330.00

USP9X/Y 抗体 (E-12) PE

sc-365353 PE
200 µg/ml
$343.00

USP9X/Y 抗体 (E-12) Alexa Fluor® 488

sc-365353 AF488
200 µg/ml
$357.00

USP9X/Y 抗体 (E-12) Alexa Fluor® 546

sc-365353 AF546
200 µg/ml
$357.00

USP9X/Y 抗体 (E-12) Alexa Fluor® 594

sc-365353 AF594
200 µg/ml
$357.00

USP9X/Y 抗体 (E-12) Alexa Fluor® 647

sc-365353 AF647
200 µg/ml
$357.00

USP9X/Y 抗体 (E-12) Alexa Fluor® 680

sc-365353 AF680
200 µg/ml
$357.00

USP9X/Y 抗体 (E-12) Alexa Fluor® 790

sc-365353 AF790
200 µg/ml
$357.00

Hi! Do you have a free sample for this ab, and especially I want lot #A1818. Please send me a quotation. Thanks a lot!

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-23

Can USP9X/Y (E-12): sc-365353 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, USP9X/Y (E-12): sc-365353 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WBTested in SHSY-5Y human neuroblastoma cells (~15-20ug/lane), dilution 1:1000 in 3% milk in TBST. Got a single clear band at expected size after very long exposure.
Date published: 2018-05-15
Rated 5 out of 5 by from 功能很出色抗体使用不错,申请的小样包装,抗体稀释比例是一比五百,WB能做几次,抗体特异性还可以,条带清晰,抗体不错
Date published: 2017-09-11
Rated 5 out of 5 by from helpfulI used it on WB, the cell is SH-SY5Y, the results are good!
Date published: 2017-08-14
Rated 4 out of 5 by from GoodEndogeous USP9X was detected in 293 cell lysates with a strong intensity.
Date published: 2017-08-08
Rated 5 out of 5 by from GoodI am able to detect endogenous USP9X in 293 cell lysates.
Date published: 2017-08-04
Rated 5 out of 5 by from goodUbiquitin controls many intracellular processes, such as signal transduction, transcriptional activation, and cell cycle progression, through the use of a broad range of enzymes that can be added or removed. Thank you for providing a well-behaved product
Date published: 2017-02-02
Rated 5 out of 5 by from Great USP9X/Y monoclonal choice for WBStrong clean bands for WB in NIH/3T3, HeLa and MCF7 whole cell lysates.
Date published: 2017-01-18
Rated 5 out of 5 by from mouse against USP9x/y antibodyI used this antibody for WB, IP and IF. It works very well.
Date published: 2016-12-15
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USP9X/Y 抗体 (E-12) is rated 4.8 out of 5 by 11.
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