USP39 CRISPR/Cas9 KO Plasmid (h): sc-406847
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP39 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP39 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP39 CRISPR/Cas9 KO Plasmid (h) | sc-406847 | 20 µg | $397.00 |
Overview
USP39 (also known as SAD1 homolog) encodes a spliceosome-associated factor that is essential for pre-mRNA splicing despite lacking deubiquitinase catalytic activity. It contributes to assembly and function of the U4/U6·U5 tri-snRNP and supports proper intron removal, linking USP39 to global transcriptome regulation and cell-cycle progression. Disruption of USP39 perturbs RNA processing programs that influence proliferation, genome maintenance, and stress responses, processes frequently altered in cancer and other proliferative disorders. As a result, USP39 is widely studied for its role in splicing-dependent control of gene expression and pathways governing mitotic fidelity.
USP39 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the USP39 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the USP39 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the USP39 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP39 protein expression.
This CRISPR knockout system enables efficient generation of USP39-deficient cell models for investigation of USP39 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting USP39 exon(s) critical for USP39 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple USP39 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP39 CRISPR/Cas9 KO Plasmid (h) and USP39 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the USP39 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP39 HDR Plasmid (h) and USP39 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by USP39 homology arms to support homology-directed repair at defined USP39 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.