Date published: 2025-9-7
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USP34 Antibody (3H9) - Western Blotting - Image 308631
USP34 抗体 (3H9): sc-100631. A549 (A) 和 HeLa (B) 全细胞裂解液中 USP34 表达的 Western 印迹分析.
USP34 抗体 (3H9): sc-100631. A549 (A) 和 HeLa (B) 全细胞裂解液中 USP34 表达的 Western 印迹分析.

USP34 抗体 (3H9): sc-100631

4.8(5)
寫評論提問

说明书
  • USP34 抗体 3H9 是小鼠单克隆 IgG2a κ，USP34抗体, 在4篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组USP34
  • 推荐用于 human 来源的USP34 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取USP34 (3H9): sc-100631的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 USP34 抗体 (3H9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与USP34 抗体 (3H9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 USP 抗体 包括 USP10, USP11, USP13, USP14, USP15, USP18, USP2, USP20, USP21和USP22

USP34抗体（3H9）是一种IgG2a κ小鼠单克隆USP34抗体，可通过WB、IP、IF、IHC(P)和ELISA检测人源的USP34蛋白。USP34抗体（3H9）为非偶联抗USP34抗体形式。泛素（Ub）途径涉及三个连续的酶促步骤，这些步骤促进Ub和Ub样分子与特定蛋白质底物的结合。通过使用能够添加或去除泛素的多种酶，Ub途径控制着许多细胞内过程，如信号转导、转录激活和细胞周期进程。USP34（泛素特异性肽酶34）是一种含有3395个氨基酸的蛋白质，属于肽酶C19蛋白家族。USP34在脑中的表达水平较低，作为去泛素化酶发挥作用，从泛素化蛋白质和泛素融合前体中裂解泛素残基，从而保护这些蛋白质免受蛋白酶体降解。在DNA损伤时，USP34会被ATM或ATR磷酸化。由于选择性剪接事件，USP34有两种异构体表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP34 抗体 (3H9) 参考文献:

  1. 人类和小鼠蛋白酶：基因组比较方法。  |  Puente, XS., et al. 2003. Nat Rev Genet. 4: 544-58. PMID: 12838346
  2. 22 种新型人类泛素特异性蛋白酶的克隆和酶学分析。  |  Quesada, V., et al. 2004. Biochem Biophys Res Commun. 314: 54-62. PMID: 14715245
  3. 发育中小鼠大脑的磷蛋白组分析  |  Ballif, BA., et al. 2004. Mol Cell Proteomics. 3: 1093-101. PMID: 15345747
  4. 对共济失调毛细血管扩张症（ATM）/ATM-Rad3相关（ATR）底物的蛋白质组分析发现，泛素-蛋白酶体系统是DNA损伤检查点的调节器。  |  Mu, JJ., et al. 2007. J Biol Chem. 282: 17330-4. PMID: 17478428
  5. 新发现的涉及 2p15p16.1 的微缺失综合征：通过全基因组平铺路径阵列比较基因组杂交分析发现的另一名患者，缩小了关键区域的范围。  |  de Leeuw, N., et al. 2008. J Med Genet. 45: 122-4. PMID: 18245392

订购信息

产品名称产品编号规格价格数量收藏夹

USP34 抗体 (3H9)

sc-100631
100 µg/ml
$316.00

USP34 (3H9): m-IgG Fc BP-HRP 套装

sc-539701
100 µg Ab; 10 µg BP
$354.00

USP34 (3H9): m-IgG2a BP-HRP 套装

sc-546762
100 µg Ab; 10 µg BP
$354.00

What percentage gel/ recommended transfer conditions do you suggest for a good band for USP34? I have used recommended dilution of 1:200 and see multiple bands (of similar intensity) above 250kd MW marker band.

Asked by: SMahajan
Thank you for your question. This is a very large protein, so we recommend a 8% gel for this protein. Please contact our Technical Service Department if you need further assistance with this antibody.
Answered by: Tech Service
Date published: 2019-02-28

What peptide has been used as the immunogen for this antibody?

Asked by: lms104
Thank you for your question. USP34 (3H9): sc-100631 was raised against amino acids 2396-3396 of human recombinant USP34 (accession# Q70CQ2). Please contact our technical service directly for additional information.
Answered by: SCBT Heidelberg Support
Date published: 2021-12-22

Can sc-100631: USP34 (3H9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-100631: USP34 (3H9) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great choice for Immunoperoxidase stainingAntibody has been used for Immunoperoxidase staining in published research. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Perfect!I have used it for western blot and IP in HEK 293T cell extract and it perfectly works.
Date published: 2020-10-20
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclearProduced beautiful immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human seminoma tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Positive band seen in WB in A549 whole cell lysatePositive band seen in WB in A549 whole cell lysate. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Strong band detected in WB in HeLa wholeStrong band detected in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-27
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USP34 抗体 (3H9) is rated 4.8 out of 5 by 5.
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