Date published: 2025-9-7
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USP30 Antibody (B-6) - Western Blotting - Image 344592
USP30 抗体 (B-6): sc-515235. USP30 在 MIA PaCa-2 全细胞裂解液中的表达的 Western 印迹分析.
USP30 抗体 (B-6): sc-515235. USP30 在 MIA PaCa-2 全细胞裂解液中的表达的 Western 印迹分析.

USP30 抗体 (B-6): sc-515235

5.0(5)
寫評論提問

说明书
  • USP30 抗体 B-6 是小鼠单克隆 IgG1 κ，USP30抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列167-466位于human物种的USP30的内部区域内
  • USP30 抗体 (B-6) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 USP30
  • 抗USP30抗体（B-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取USP30 (B-6): sc-515235的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是USP30 Antibody (B-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与USP30 Antibody (B-6) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外USP抗体，包括USP10, USP11, USP13, USP14, USP15, USP18, USP2, USP20, USP21, USP22, USP25, USP29, USP3, USP30, USP31和USP32

USP30抗体（B-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人源USP30。抗USP30抗体（B-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。USP30由517个氨基酸组成，主要位于线粒体中，在泛素-蛋白酶体系统中起着至关重要的作用，泛素-蛋白酶体系统是控制蛋白质降解和细胞稳态的关键调节途径。USP30对于维持线粒体的功能和完整性至关重要，因为USP30催化泛素C端硫酯向游离泛素和硫醇的转化，从而调节线粒体蛋白质的更新。编码USP30的基因位于人类第12号染色体上，该染色体包含1100多个基因，约占人类基因组的4.5%。USP30的功能对于多种细胞过程至关重要，包括信号转导和细胞周期调节，因此USP30成为细胞生物学和疾病机制研究的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP30 抗体 (B-6) 参考文献:

  1. 从基因组角度看人类蛋白酶。  |  Southan, C. 2001. FEBS Lett. 498: 214-8. PMID: 11412860
  2. 人类和小鼠蛋白酶：基因组比较方法。  |  Puente, XS., et al. 2003. Nat Rev Genet. 4: 544-58. PMID: 12838346
  3. 22 种新型人类泛素特异性蛋白酶的克隆和酶学分析。  |  Quesada, V., et al. 2004. Biochem Biophys Res Commun. 314: 54-62. PMID: 14715245
  4. 线粒体外膜中的一种去泛素化酶 USP30 对线粒体形态的调节。  |  Nakamura, N. and Hirose, S. 2008. Mol Biol Cell. 19: 1903-11. PMID: 18287522
  5. 去泛素化酶对泛素依赖过程的调节。  |  Wilkinson, KD. 1997. FASEB J. 11: 1245-56. PMID: 9409543

订购信息

产品名称产品编号规格价格数量收藏夹

USP30 抗体 (B-6)

sc-515235
200 µg/ml
$316.00

USP30 (B-6): m-IgG Fc BP-HRP 套装

sc-531321
200 µg Ab; 10 µg BP
$354.00

USP30 (B-6): m-IgGκ BP-HRP 套装

sc-524984
200 µg Ab, 40 µg BP
$354.00

USP30 (B-6): m-IgG1 BP-HRP 套装

sc-544484
200 µg Ab; 20 µg BP
$354.00

USP30 抗体 (B-6) AC

sc-515235 AC
500 µg/ml, 25% agarose
$416.00

USP30 抗体 (B-6) HRP

sc-515235 HRP
200 µg/ml
$316.00

USP30 抗体 (B-6) FITC

sc-515235 FITC
200 µg/ml
$330.00

USP30 抗体 (B-6) PE

sc-515235 PE
200 µg/ml
$343.00

USP30 抗体 (B-6) Alexa Fluor® 488

sc-515235 AF488
200 µg/ml
$357.00

USP30 抗体 (B-6) Alexa Fluor® 546

sc-515235 AF546
200 µg/ml
$357.00

USP30 抗体 (B-6) Alexa Fluor® 594

sc-515235 AF594
200 µg/ml
$357.00

USP30 抗体 (B-6) Alexa Fluor® 647

sc-515235 AF647
200 µg/ml
$357.00

USP30 抗体 (B-6) Alexa Fluor® 680

sc-515235 AF680
200 µg/ml
$357.00

USP30 抗体 (B-6) Alexa Fluor® 790

sc-515235 AF790
200 µg/ml
$357.00

Do you know if this antibody will recognize mouse USP30?

Asked by: MJJ1979
Thank you for your question. USP30 Antibody (B-6) is only recommended for detection of USP30 of human origin by WB, IP, IF and ELISA. Based on our tests with this product we do not recommend it for use with murine cell or tissue samples.
Answered by: Tech Support Europe
Date published: 2022-08-17

Hello, How can I get a FREE 10 µg sample of USP30 (B-6): sc-515235? I've been trying an antibody from Abcam for a western blot application but had poor results. I would like to give your antibody a try and if it works we can order a full size.

Asked by: Miluska
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-09-27

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from Good for Western blotAntibody works for Western blot with Hek293 cell lysate; expression eliminated in CRISPR/Cas9-mediated USP30 knockout cells in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Great antibody for wbA good reasult in Western blot. Used at dilution 1:1000, a strong band with predicted size was detected in HeLa cells.
Date published: 2017-07-21
Rated 5 out of 5 by from ExcellentExcellent performance in Western blot. Used at dilution 1:2000, after blocking with milk. Single band with predicted size was detected in HeLa cell lysates. Also HeLa cell fractionation followed by Western blot revealed a correct mitochondrial localization.
Date published: 2017-07-04
Rated 5 out of 5 by from strong signal and outstanding resultI used this antibody in Western blotting with 1:1000 dilution. WB got a strong signal, we are very pleasure.
Date published: 2017-02-12
Rated 5 out of 5 by from Great antibody for WVNice clean WB bands produced with USP30 Antibody (B-6)
Date published: 2017-01-12
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USP30 抗体 (B-6) is rated 5.0 out of 5 by 5.
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