UGT8 CRISPR/Cas9 KO Plasmid (m2): sc-423604-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UGT8 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UGT8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UGT8 CRISPR/Cas9 KO Plasmid (m2) | sc-423604-KO-2 | 20 µg | $397.00 |
Overview
Ugt8a encodes UDP-galactose:ceramide galactosyltransferase (UGT8), a Golgi-resident glycosyltransferase that catalyzes formation of galactosylceramide, a key precursor for sulfatide and other galactolipids. This enzymatic step supports sphingolipid and myelin lipid biosynthesis, influencing membrane microdomain composition, axon–glia interactions, and oligodendrocyte maturation in the mouse nervous system. Altered galactolipid balance has been associated with dysregulated myelination and neuroinflammatory phenotypes, making UGT8 a relevant node for studying lipid-driven neuronal and glial biology. UGT8 activity is also used to interrogate broader ceramide flux and glycosphingolipid pathway remodeling under developmental or stress conditions.
UGT8 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Ugt8a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ugt8a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ugt8a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UGT8 protein expression.
This CRISPR knockout system enables efficient generation of Ugt8a-deficient cell models for investigation of UGT8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ugt8a exon(s) critical for UGT8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ugt8a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UGT8 CRISPR/Cas9 KO Plasmid (m) and UGT8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ugt8a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UGT8 HDR Plasmid (m) and UGT8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ugt8a homology arms to support homology-directed repair at defined Ugt8a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.