UGT2B15 CRISPR/Cas9 KO Plasmid (h): sc-405610
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UGT2B15 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UGT2B15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UGT2B15 CRISPR/Cas9 KO Plasmid (h) | sc-405610 | 20 µg | $397.00 |
Overview
UGT2B15 encodes a human UDP-glucuronosyltransferase that catalyzes phase II glucuronidation of endogenous hormones and diverse xenobiotics, increasing their solubility for elimination. As an ER-membrane enzyme in the UGT2 family, it contributes to hepatic and extrahepatic metabolic clearance and shapes intracellular exposure to bioactive steroids through conjugation-dependent inactivation. Variation in UGT2B15 activity can shift androgen and other steroid homeostasis, influencing endocrine signaling networks and downstream transcriptional programs. Altered expression or polymorphisms have been investigated in the context of hormone-dependent cancers and interindividual differences in drug metabolism and toxicity phenotypes.
UGT2B15 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UGT2B15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UGT2B15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UGT2B15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UGT2B15 protein expression.
This CRISPR knockout system enables efficient generation of UGT2B15-deficient cell models for investigation of UGT2B15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UGT2B15 exon(s) critical for UGT2B15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UGT2B15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UGT2B15 CRISPR/Cas9 KO Plasmid (h) and UGT2B15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UGT2B15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UGT2B15 HDR Plasmid (h) and UGT2B15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UGT2B15 homology arms to support homology-directed repair at defined UGT2B15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.