UFD2 Antibody (7): sc-136115
- UFD2 Antibody (7) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
- raised against amino acids 1034-1241 of UFD2 of human origin
- recommended for detection of UFD2 of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UFD2 Antibody (7). This work is in progress.
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UFD2 Antibody (7) is a mouse monoclonal IgG1 antibody that detects UFD2 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. UFD2, also known as ubiquitin conjugation factor E4 (UBE4B), plays a crucial role in the ubiquitin-proteasome system by binding to ubiquitin moieties of preformed conjugates and facilitating the assembly of ubiquitin chains in collaboration with E1, E2, and E3 enzymes. This process is vital for regulating protein degradation, which is essential for maintaining cellular homeostasis and responding to stress. During apoptosis, UFD2 undergoes proteolytic cleavage at Asp 123 by caspase-6 and granzyme B, with a significantly lower cleavage efficiency at Asp 109 by caspase-3 and caspase-7. UFD2′s ability to modulate protein turnover is particularly important in eukaryotic cells, as research has linked UFD2 to cell survival under stress conditions in yeast, suggesting that E4-dependent proteolysis pathways are utilized for various cellular functions across species. UFD2 is predominantly expressed in the ovary, testis, heart, and skeletal muscle, highlighting UFD2′s potential significance in reproductive and muscular health.
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UFD2 Antibody (7) References:
- A Ufd2/D4Cole1e chimeric protein and overexpression of Rbp7 in the slow Wallerian degeneration (WldS) mouse. | Conforti, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 11377-82. PMID: 11027338
- U-box proteins as a new family of ubiquitin ligases. | Hatakeyama, S. and Nakayama, KI. 2003. Biochem Biophys Res Commun. 302: 635-45. PMID: 12646216
- Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage. | Saeki, Y., et al. 2004. Biochem Biophys Res Commun. 320: 840-5. PMID: 15240124
- Ufd2, a novel autoantigen in scleroderma, regulates sister chromatid separation. | Spinette, S., et al. 2004. Cell Cycle. 3: 1638-44. PMID: 15611659
- The two human homologues of yeast UFD2 ubiquitination factor, UBE4A and UBE4B, are located in common neuroblastoma deletion regions and are subject to mutations in tumours. | Carén, H., et al. 2006. Eur J Cancer. 42: 381-7. PMID: 16386891
- Ubiquitin chain elongation enzyme Ufd2 regulates a subset of Doa10 substrates. | Liu, C., et al. 2010. J Biol Chem. 285: 10265-72. PMID: 20159987
- The yeast E4 ubiquitin ligase Ufd2 interacts with the ubiquitin-like domains of Rad23 and Dsk2 via a novel and distinct ubiquitin-like binding domain. | Hänzelmann, P., et al. 2010. J Biol Chem. 285: 20390-8. PMID: 20427284
- Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase. | Liu, C., et al. 2011. J Biol Chem. 286: 43660-43667. PMID: 22045814
- E4-Ubiquitin ligase Ufd2 stabilizes Yap8 and modulates arsenic stress responses independent of the U-box motif. | Ferreira, RT., et al. 2015. Biol Open. 4: 1122-31. PMID: 26276098
- E4 ubiquitin ligase promotes mitofusin turnover and mitochondrial stress response. | Anton, V., et al. 2023. Mol Cell. 83: 2976-2990.e9. PMID: 37595558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFD2 Antibody (7) | sc-136115 | 50 µg/0.5 ml | $322.00 |