Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ubr4 CRISPR/Cas9 KO Plasmid (h): sc-408481
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ubr4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ubr4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ubr4 Antibody (21-Y): sc-100615
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ubr4 CRISPR/Cas9 KO Plasmid (h) | sc-408481 | 20 µg | $397.00 |
Overview
Human UBR4 encodes the E3 ubiquitin ligase Ubr4, a large cytosolic protein that contributes to ubiquitin-dependent protein quality control and turnover. Ubr4 is linked to the N-end rule pathway and broader proteostasis programs that influence protein stability, trafficking, and stress-adaptive remodeling of the cellular proteome. Through these roles, UBR4 can affect processes such as endocytosis, cell-cycle–associated protein regulation, and responses to proteotoxic stress. Dysregulation of ubiquitin signaling and proteostasis has been associated with neurodegenerative phenotypes and cancer-related cellular states, making UBR4 a useful node for mechanistic studies of ubiquitin pathway wiring.
Ubr4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBR4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBR4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBR4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ubr4 protein expression.
This CRISPR knockout system enables efficient generation of UBR4-deficient cell models for investigation of Ubr4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBR4 exon(s) critical for Ubr4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBR4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ubr4 CRISPR/Cas9 KO Plasmid (h) and Ubr4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBR4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ubr4 HDR Plasmid (h) and Ubr4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBR4 homology arms to support homology-directed repair at defined UBR4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.