Date published: 2025-9-6
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UBC12 Antibody (L-34) - Western Blotting - Image 35081
UBC12 抗体 (L-34): sc-100608. 拉莫斯全细胞裂解液中 UBC12 表达的 Western 印迹分析.
UBC12 抗体 (L-34): sc-100608. 拉莫斯全细胞裂解液中 UBC12 表达的 Western 印迹分析.

UBC12 抗体 (L-34): sc-100608

5.0(6)
寫評論提問

说明书
  • UBC12抗体(L-34)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组UBC12
  • 推荐用于 human 来源的UBC12 WB, IP, IF 和 ELISA检测
  • 目前，我们还没有完成UBC12 抗体 (L-34)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 UBC 抗体 包括 UBC13, UBE2NL, UBC12, Ubc2, UBC8和UBC9

泛素化是一种重要的机制，通过该机制，三类酶协同作用，将短命或异常蛋白质作为降解目标。参与泛素化的三类酶是泛素激活酶（E1s）、泛素结合酶（E2s）和泛素蛋白连接酶（E3s）。UBC12，也称为UBE2M（泛素结合酶E2M）、hUbc12或UBC-RS2，是E2泛素结合酶家族的183个氨基酸成员。UBC12与NEDD8（神经前体细胞表达，发育下调8）相关联，NEDD8是一种类似泛素的蛋白质。通过这种相互作用，UBC12促进NEDD8附着到目标降解蛋白质上。由于UBC12能够控制NEDD8与细胞蛋白质的结合，因此认为UBC12在细胞增殖事件中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

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产品名称产品编号规格价格数量收藏夹

UBC12 抗体 (L-34)

sc-100608
100 µg/ml
$333.00

I am using UBC12 (L-34): sc-100608 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great monoclonal antibodyStrong band observed with little to no background with UBC12 Antibody (L-34)
Date published: 2017-02-08
Rated 5 out of 5 by from WB publication with human THPWB publication with human THP-1 cells.
Date published: 2015-06-11
Rated 5 out of 5 by from Worked well for Western blot with human transfectedWorked well for Western blot with human transfected lysates. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear stainingBeautiful immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Positive Western blot data obtained usingPositive Western blot data obtained using Ramos whole cell lysates. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Worked great for WB with Jurkat whole cell lysatesWorked great for WB with Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-04
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UBC12 抗体 (L-34) is rated 5.0 out of 5 by 6.
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