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UBC12 Antibody (L-34) - Western Blotting - Image 35081
UBC12 抗体 (L-34): sc-100608. 拉莫斯全细胞裂解液中 UBC12 表达的 Western 印迹分析.
UBC12 抗体 (L-34): sc-100608. 拉莫斯全细胞裂解液中 UBC12 表达的 Western 印迹分析.

UBC12 抗体 (L-34): sc-100608

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说明书
  • UBC12抗体(L-34)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组UBC12
  • 推荐用于 human 来源的UBC12 WB, IP, IF 和 ELISA检测
  • 目前，我们还没有完成UBC12 抗体 (L-34)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

额外UBC抗体，包括UBC13, UBE2NL, UBC12, Ubc2, UBC8和UBC9

泛素化是一种重要的机制，通过该机制，三类酶协同作用，将短命或异常蛋白质作为降解目标。参与泛素化的三类酶是泛素激活酶（E1s）、泛素结合酶（E2s）和泛素蛋白连接酶（E3s）。UBC12，也称为UBE2M（泛素结合酶E2M）、hUbc12或UBC-RS2，是E2泛素结合酶家族的183个氨基酸成员。UBC12与NEDD8（神经前体细胞表达，发育下调8）相关联，NEDD8是一种类似泛素的蛋白质。通过这种相互作用，UBC12促进NEDD8附着到目标降解蛋白质上。由于UBC12能够控制NEDD8与细胞蛋白质的结合，因此认为UBC12在细胞增殖事件中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

UBC12 抗体 (L-34) 参考文献:

  1. 显性阴性的 UBC12 突变体能螯合 NEDD8 并抑制 NEDD8 在体内的结合。  |  Wada, H., et al. 2000. J Biol Chem. 275: 17008-15. PMID: 10828074
  2. DCN1-UBC12 蛋白-蛋白相互作用的高亲和性拟肽抑制剂  |  Zhou, H., et al. 2018. J Med Chem. 61: 1934-1950. PMID: 29438612
  3. 验证NEDD8结合酶UBC12作为肺癌的新治疗靶点。  |  Li, L., et al. 2019. EBioMedicine. 45: 81-91. PMID: 31208947
  4. 靶向DCN1-UBC12蛋白-蛋白相互作用，调节Neddylation途径。  |  Zhou, H., et al. 2020. Adv Exp Med Biol. 1217: 349-362. PMID: 31898237
  5. UBC12介导的SREBP-1 neddylation恶化转移性肿瘤预后。  |  Heo, MJ., et al. 2020. Int J Cancer. 147: 2550-2563. PMID: 32449166
  6. 开发苯基三唑硫醇衍生物作为DCN1-UBC12相互作用的高效抑制剂。  |  Zhou, W., et al. 2021. Eur J Med Chem. 217: 113326. PMID: 33756127
  7. 发现强效和选择性 2-(苄硫基)嘧啶型 DCN1-UBC12 抑制剂，发挥抗心肌纤维化作用  |  He, ZX., et al. 2022. J Med Chem. 65: 163-190. PMID: 34939411
  8. 评估新型DCN1-UBC12相互作用抑制剂HZX-960作为肝纤维化的潜在抗纤维化化合物的效果。  |  Zhou, W., et al. 2022. Biochem Cell Biol. 100: 309-324. PMID: 35544948
  9. Arctigenin抑制UBC12酶活性，减少癌细胞。  |  Chen, YF., et al. 2023. Acta Pharmacol Sin. 44: 661-669. PMID: 36138144
  10. NNMT/1-MNA通过靶向UBC12/Cullin-1介导的P27蛋白降解促进乳腺癌细胞周期的进展。  |  Ma, Y., et al. 2024. Adv Sci (Weinh). 11: e2305907. PMID: 38126621

订购信息

产品名称产品编号规格价格数量收藏夹

UBC12 抗体 (L-34)

sc-100608
100 µg/ml
$339.00

I am using UBC12 (L-34): sc-100608 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by jenniferc from Great monoclonal antibodyStrong band observed with little to no background with UBC12 Antibody (L-34)
Date published: 2017-02-08
Rated 5 out of 5 by QZ from WB publication with human THPWB publication with human THP-1 cells.
Date published: 2015-06-11
Rated 5 out of 5 by LM from Worked well for Western blot with human transfectedWorked well for Western blot with human transfected lysates. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by QZ from Beautiful immunofluorescence nuclear stainingBeautiful immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by NZ from Positive Western blot data obtained usingPositive Western blot data obtained using Ramos whole cell lysates. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by CJ from Worked great for WB with Jurkat whole cell lysatesWorked great for WB with Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-04
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UBC12 抗体 (L-34) is rated 5.0 out of 5 by 6.
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