UBC CRISPR/Cas9 KO Plasmid (m): sc-418924
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBC CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBC CRISPR/Cas9 KO Plasmid (m) | sc-418924 | 20 µg | $397.00 |
Overview
Mouse Ubc encodes polyubiquitin-C (UBC), a major source of ubiquitin monomers required for ATP-dependent protein turnover by the ubiquitin–proteasome system. UBC supports ubiquitination-driven regulation of cell cycle progression, DNA damage responses, stress adaptation, and inflammatory signaling by controlling the stability and activity of key pathway components. Because ubiquitin homeostasis buffers proteotoxic stress and shapes signaling output, altered ubiquitin supply and proteostasis imbalance are frequently examined in models of neurodegeneration, cancer-relevant growth control, and immune dysregulation. Ubc is therefore commonly used to interrogate proteasome-dependent quality control, ubiquitin chain dynamics, and crosstalk with autophagy and stress response pathways in mouse cells.
UBC CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ubc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ubc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ubc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBC protein expression.
This CRISPR knockout system enables efficient generation of Ubc-deficient cell models for investigation of UBC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ubc exon(s) critical for UBC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ubc genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBC CRISPR/Cas9 KO Plasmid (m) and UBC CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ubc locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBC HDR Plasmid (m) and UBC HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ubc homology arms to support homology-directed repair at defined Ubc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.