Date published: 2025-9-7
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U1 snRNP 70 Antibody (C-3) - Western Blotting - Image 373590
U1 snRNP 70 抗体 (C-3): sc-390899. Hep G2 (A), Jurkat (B) 和 RAW 264. 7 (C) 全细胞裂解液中 U1 snRNP 70 表达的 Western 印迹分析.
U1 snRNP 70 抗体 (C-3): sc-390899. Hep G2 (A), Jurkat (B) 和 RAW 264. 7 (C) 全细胞裂解液中 U1 snRNP 70 表达的 Western 印迹分析.

U1 snRNP 70 抗体 (C-3): sc-390899

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说明书
  • U1 snRNP 70 抗体 C-3 是小鼠单克隆 IgG1 κ，U1 snRNP 70抗体, 在24篇文献中引用，规格为200 µg/ml
  • 与human的183-280氨基酸残基的U1 snRNP 70
  • U1 snRNP 70 抗体 (C-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 U1 snRNP 70
  • 抗U1 snRNP 70抗体（C-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取U1 snRNP 70 (C-3): sc-390899的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 U1 snRNP 70 抗体 (C-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与U1 snRNP 70 抗体 (C-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

U1 snRNP 70抗体（C-3）是一种IgG1 κ小鼠单克隆U1 snRNP 70抗体（也被称为RU17抗体、小核核糖核蛋白U1亚基70抗体、U1-70K抗体、U1小核核糖核蛋白70 KDa抗体、U1 SnRNP 70 KDa抗体、RNPU1Z抗体、SNRP70抗体、RPU1抗体、Snp1抗体、小核核糖核蛋白70kDa（RNP抗原）抗体、U170K抗体、U1RNP抗体、U1AP1或U1AP抗体），它可以通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（石蜡）和酶联免疫吸附试验检测小鼠、大鼠和人类来源的U1 snRNP 70蛋白。U1 snRNP 70抗体（C-3）既可作为非偶联形式的抗U1 snRNP 70抗体使用，也可作为多种偶联形式的抗U1 snRNP 70抗体使用，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。U1小核核糖核蛋白（U1 snRNP 70或U1 70）是RNA剪接体的一个组成部分，剪接体是一种蛋白质复合物，用于从前体信使RNA（pre-mRNA）中精确切除内含子。U1 snRNP 70特异性地与U1 snRNA（小核RNA）发夹1的单链环结合。与其他snRNPs一样，U1 snRNP 70含有一个位于蛋白质中央的80-90个氨基酸的RNA结合域，这对于体外特异的U1 snRNA结合既是必要的也是充分的。这种独立于ATP发生的相互作用对于前体mRNA剪接途径的承诺是至关重要的，因为它促进了其他蛋白质与剪接体的结合。在有丝分裂开始时，U1 snRNP 70弥散地定位于细胞质中，随着有丝分裂进入末期，U1 snRNP 70在子核中积累。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

U1 snRNP 70 抗体 (C-3) 参考文献:

  1. 系统性硬化症患者体内抗 U1RNP 抗体的分布和抗原特异性。  |  Ihn, H., et al. 1999. Clin Exp Immunol. 117: 383-7. PMID: 10444274
  2. U1 RNP 抗体特异性在人类自身免疫抗血清中的先后顺序显示，U1-70 kd 和 B'/B 蛋白是主要的 U1 RNP 免疫原。  |  Greidinger, EL. and Hoffman, RW. 2001. Arthritis Rheum. 44: 368-75. PMID: 11229468
  3. 凋亡的 U1-70 kd 与完整形态的 U1-70-kd 分子在抗原性上截然不同。  |  Greidinger, EL., et al. 2002. Arthritis Rheum. 46: 1264-9. PMID: 12115232
  4. 重组 70-kd 蛋白和 U1 RNA 与混合结缔组织病患者血清中的抗 U1 RNP 自身抗体结合产生的新构象表位。  |  Murakami, A., et al. 2002. Arthritis Rheum. 46: 3273-82. PMID: 12483732
  5. 人类 parvovirus B19 非结构蛋白 NS1 增强了 70 kDa U1-snRNP 自身抗原的裂解表达。  |  Tzang, BS., et al. 2010. J Biomed Sci. 17: 40. PMID: 20500824
  6. 人类 U1-70K 核糖核蛋白抗原蛋白与 U1 小核 RNA 环 I 的直接, 序列特异性结合。  |  Surowy, CS., et al. 1989. Mol Cell Biol. 9: 4179-86. PMID: 2531275
  7. U1 RNA 的一个特定 31 核苷酸结构域直接与 70K 小核核糖核蛋白成分相互作用。  |  Query, CC., et al. 1989. Mol Cell Biol. 9: 4872-81. PMID: 2532301
  8. 体外组装 U1 snRNPs。  |  Hamm, J., et al. 1987. EMBO J. 6: 3479-85. PMID: 2962858
  9. 肝细胞癌患者体内小核核糖核蛋白 U1 亚基 70 的临床意义。  |  Jiang, D., et al. 2024. PeerJ. 12: e16876. PMID: 38500533
  10. 解密针对 U1-snRNP 复合物中 RNP-A、-C 和 70 kDa 成分的自身抗体的反应性：'双倍或全无'？  |  Bertin, D., et al. 2024. Biomedicines. 12: PMID: 39062124
  11. 通过体外组装研究 U1 小核核糖核蛋白。  |  Wieben, ED., et al. 1983. J Cell Biol. 96: 1751-5. PMID: 6133878
  12. 在有丝分裂和子细胞核组装过程中，剪接 snRNPs 与盘绕体和染色质颗粒之间的相互作用存在差异。  |  Ferreira, JA., et al. 1994. J Cell Biol. 126: 11-23. PMID: 8027171

订购信息

产品名称产品编号规格价格数量收藏夹

U1 snRNP 70 抗体 (C-3)

sc-390899
200 µg/ml
$316.00

U1 snRNP 70 (C-3): m-IgGκ BP-HRP 套装

sc-523724
200 µg Ab, 40 µg BP
$354.00

U1 snRNP 70 (C-3): m-IgG1 BP-HRP 套装

sc-533606
200 µg Ab; 20 µg BP
$354.00

U1 snRNP 70 抗体 (C-3) AC

sc-390899 AC
500 µg/ml, 25% agarose
$416.00

U1 snRNP 70 抗体 (C-3) HRP

sc-390899 HRP
200 µg/ml
$316.00

U1 snRNP 70 抗体 (C-3) FITC

sc-390899 FITC
200 µg/ml
$330.00

U1 snRNP 70 抗体 (C-3) PE

sc-390899 PE
200 µg/ml
$343.00

U1 snRNP 70 抗体 (C-3) Alexa Fluor® 488

sc-390899 AF488
200 µg/ml
$357.00

U1 snRNP 70 抗体 (C-3) Alexa Fluor® 546

sc-390899 AF546
200 µg/ml
$357.00

U1 snRNP 70 抗体 (C-3) Alexa Fluor® 594

sc-390899 AF594
200 µg/ml
$357.00

U1 snRNP 70 抗体 (C-3) Alexa Fluor® 647

sc-390899 AF647
200 µg/ml
$357.00

U1 snRNP 70 抗体 (C-3) Alexa Fluor® 680

sc-390899 AF680
200 µg/ml
$357.00

U1 snRNP 70 抗体 (C-3) Alexa Fluor® 790

sc-390899 AF790
200 µg/ml
$357.00

U1 snRNP 70 (C-3) 中和勝肽

sc-390899 P
100 µg/0.5 ml
$68.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with U1 snRNP 70 Antibody (C-3): sc-390899?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-31
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Rated 5 out of 5 by from It tells what it should be!We had this sample test and it was really great for U1 snRNP 70k! Our lab has one more of goat antibody, but not as good as this one.
Date published: 2021-11-17
Rated 4 out of 5 by from ok antibody, wrong info on datasheetAntibody works ok. The molecular weight of U1-70k is ~53kda; the kda reported on the datasheet of 70kda is incorrect.
Date published: 2021-08-11
Rated 5 out of 5 by from strong specific bandsI used this this ab for WB in mESC (1:500 dilution, 20 ug of total protein). Works really well (see attached)
Date published: 2018-03-29
Rated 5 out of 5 by from result satisfiedIn WB experiment,1/600 dilution in Jurkat whole cell lysates. I found that it was very good and the signals were very strong.
Date published: 2017-10-25
Rated 5 out of 5 by from snRNP 70 reviewI used the snRNP 70 (C-3) antibody (1:500 dilution) for western blotting and got a very good signal.
Date published: 2017-08-25
Rated 5 out of 5 by from works well in IP, WBThis antibody works well in IP and WB with HeLa cells. The signal band in WB, IP are clear and specific. The IP performed by DMP cross-linking method with protein G from G&E.
Date published: 2017-06-23
Rated 5 out of 5 by from Works good in WB!Gives a single band at the expected size in WB. Dilution 1:500, HLE cells 10µg/lane
Date published: 2017-03-08
Rated 5 out of 5 by from Good AntibodyU1 snRNP 70 Antibody is really great, the analysis results of Western blot were very strong and clear. We will introduce the with partners
Date published: 2017-02-12
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U1 snRNP 70 抗体 (C-3) is rated 4.9 out of 5 by 12.
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