Type I 4-phosphatase Double Nickase Plasmid (h): sc-404750-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Type I 4-phosphatase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Type I 4-phosphatase Double Nickase Plasmid (h) and Type I 4-phosphatase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting INPP4A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Type I 4-phosphatase Antibody (E-2): sc-390549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Type I 4-phosphatase Double Nickase Plasmid (h) | sc-404750-NIC | 20 µg | $410.00 | |||
Type I 4-phosphatase Double Nickase Plasmid (h2) | sc-404750-NIC-2 | 20 µg | $410.00 |
INPP4A encodes type I 4-phosphatase, an inositol polyphosphate 4-phosphatase that hydrolyzes phosphatidylinositol 3,4-bisphosphate to phosphatidylinositol 3-phosphate, reshaping membrane phosphoinositide pools. Through this activity it modulates PI3K-dependent signaling dynamics, influencing recruitment of PH-domain effectors, endosomal membrane identity, and downstream control of cell growth and survival programs. INPP4A function intersects with receptor-mediated signaling and vesicular trafficking processes that coordinate nutrient sensing and stress responses. Altered regulation of INPP4A and PI(3,4)P2 turnover has been linked in the literature to dysregulated signaling networks relevant to cancer biology and neurobiology, supporting mechanistic studies of pathway rewiring.
Type I 4-phosphatase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the INPP4A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within INPP4A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt INPP4A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of INPP4A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.