Date published: 2025-9-5
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twist Antibody (Twist2C1a) - Western Blotting - Image 366608
twist 抗体 (Twist2C1a): sc-81417. 对 SH-SY5Y 核提取物 (A) 和 3T3-L1 全细胞裂解液 (B) 中 twist 表达的 Western 印迹分析.
twist 抗体 (Twist2C1a): sc-81417. 对 SH-SY5Y 核提取物 (A) 和 3T3-L1 全细胞裂解液 (B) 中 twist 表达的 Western 印迹分析.

twist 抗体 (Twist2C1a): sc-81417

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说明书
  • twist 抗体 Twist2C1a 是小鼠单克隆 IgG1 κ，twist抗体, 在251篇文献中引用，规格为100 µg/ml
  • 针对与human twist C-terminus附近区域相对应的重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的twist WB, IP, IF 和 FCM检测
  • 关于如何获取twist (Twist2C1a): sc-81417的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是twist Antibody (Twist2C1a) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与twist Antibody (Twist2C1a) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Twist抗体（Twist2C1a）是一种IgG1 κ小鼠单克隆Twist抗体（也称为Twist1抗体），可通过WB, IP, IF 和 FCM检测小鼠、大鼠和人类来源的Twist蛋白。Twist抗体（Twist2C1a）为非偶联的抗Twist抗体。肌源性决定因子家族的成员是基本螺旋-环-螺旋（bHLH）蛋白，可分为两类。A类蛋白包括普遍表达的E-box结合因子E12/E47、ITF2和HEB（BETA1或HTF4）。B类蛋白如MyoD、肌细胞生成素和NeuroD（BETA2）是瞬时表达的，且组织分布更为有限。A类蛋白与B类蛋白异二聚以激活DNA转录。与这些起正向作用的因子相对立的是一组专门起显性负调控作用的蛋白质。肌肉组织来源于胚胎中胚层的一组细胞。新型基本螺旋-环-螺旋（bHLH）转录因子Twist是中胚层分化和肌生成的假定调节因子。Twist在整个上皮生骨节中表达，但在肌节中不表达。Twist需要与E蛋白二聚化，并抑制肌源性调节因子。它已被证明是肌节时间和空间形成的调节因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

twist 抗体 (Twist2C1a) 参考文献:

  1. Saethre-Chotzen突变导致TWIST蛋白降解或核定位受损。  |  El Ghouzzi, V., et al. 2000. Hum Mol Genet. 9: 813-9. PMID: 10749989
  2. 前列腺癌中 TWIST 的上调及其作为治疗靶点的意义。  |  Kwok, WK., et al. 2005. Cancer Res. 65: 5153-62. PMID: 15958559
  3. TWIST 和 E-cadherin 表达在前列腺癌转移进展中的意义。  |  Yuen, HF., et al. 2007. Histopathology. 50: 648-58. PMID: 17394502
  4. 视网膜母细胞瘤细胞衍生的 Twist 蛋白可促进调节性 T 细胞的发育。  |  Zhang, R., et al. 2021. Cancer Immunol Immunother. 70: 1037-1048. PMID: 33108472
  5. 基本螺旋环螺旋蛋白 NeuroD 将爪蟾外胚层转化为神经元。  |  Lee, JE., et al. 1995. Science. 268: 836-44. PMID: 7754368
  6. 一种新型碱性螺旋-环-螺旋转录因子对胰岛素基因的组织特异性调控。  |  Naya, FJ., et al. 1995. Genes Dev. 9: 1009-19. PMID: 7774807
  7. 螺旋环-螺旋二聚化亲和力的决定因素。SCL/tal的随机突变分析。  |  Goldfarb, AN., et al. 1996. J Biol Chem. 271: 2683-8. PMID: 8576241
  8. bHLH 蛋白 Twist 对成肌细胞 bHLH 和 MEF2 转录因子的抑制。  |  Spicer, DB., et al. 1996. Science. 272: 1476-80. PMID: 8633239
  9. 扭转：果蝇的成肌开关。  |  Baylies, MK. and Bate, M. 1996. Science. 272: 1481-4. PMID: 8633240
  10. Id2的表达随着人类髓系细胞的分化而增加。  |  Ishiguro, A., et al. 1996. Blood. 87: 5225-31. PMID: 8652837
  11. 取代基本区的碱性氨基酸可稳定 E12 同源二聚体的 DNA 结合。  |  Vitola, SJ., et al. 1996. Nucleic Acids Res. 24: 1921-7. PMID: 8657575
  12. 小鼠胚胎发育中的Twist蛋白。  |  Gitelman, I. 1997. Dev Biol. 189: 205-14. PMID: 9299114

订购信息

产品名称产品编号规格价格数量收藏夹

twist 抗体 (Twist2C1a)

sc-81417
100 µg/ml
$333.00

twist (Twist2C1a): m-IgG1 BP-HRP 套装

sc-542627
100 µg Ab; 20 µg BP
$354.00

Which is the best condition for the antibody 1:200, 1:500 or 1:1000 , I'm going to use whole lysate? Have you tried it on MCF-7?

Asked by: Nicoletta
Thank you for your question. The ideal dilution depends on your particular samples and experimental setup, so it is best to try what works best in your lab. Twist Antibody (Twist2C1a): sc-81417 is recommended for detection of twist of mouse, rat and human origin by WB, IP, IF and FCM and detects its target in MCF-7 cells as well.
Answered by: Tech Support Europe
Date published: 2023-01-16

Does this twist Ab recognize TWIST1 or TWIST2?

Asked by: Marvel Luo
Thank you for your question. This antibody is specific for TWIST1, and does not cross-react with TWIST2.
Answered by: Technical Support
Date published: 2021-11-23

Can this work in Drosophila? 

Asked by: Rachelpi
Thank you for your question. This antibody has not yet been tested in Drosophila, so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-10-30

Dear Sir/ madame, I would like to ask if the sc-81417 is available as conjugate with Alexa-Fluor- 647. Thanks in advance Maria

Asked by: Maria74
Thank you for your question. This antibody is not available conjugated to AF647 at this time. For accurate availability, shipping and pricing information regarding our products, please contact Customer service or your local distributor.
Answered by: Tech Service
Date published: 2019-09-23

Does it pull down TWIST1 in ChIP?

Asked by: Anirudh
Yes, this antibody is specific for TWIST1 and we are aware of several publications citing use of this antibody for ChIP applications. These include PMID’s: 24491388, 24848707 and 22668877.
Answered by: Technical Support
Date published: 2018-12-14

I am wondering if this antibody also have cross-reactivity with TWIST2 or not, looks like most antibodies of different company have ?

Asked by: kidarime
This antibody is specific for TWIST1 and does not cross-react with TWIST2. The antibody has been tested against specific transfected lysates and only showed cross-reactivity with the TWIST1 lysate. We will update our product information and publish the data shortly for this antibody!
Answered by: Technical Support
Date published: 2018-12-14

I need to use an antibody to detect twist porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2019-10-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary for Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-16
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Rated 1 out of 5 by from No bands in WesternIt is disappointing that no bands were visible in EMT-induced cells.
Date published: 2024-08-18
Rated 4 out of 5 by from Works for IF and IHCIHC: dilution 1/100, pH9, autoclave (120 ℃, 5 min) IF (immunocytochemistry of fibroblasts): dilution 1/50
Date published: 2024-06-25
Rated 5 out of 5 by from Great specific antibody for human and mouse TWIST1Antibody is specific for TWIST1 and not for TWIST2: Lane 2: human TWIST1 transfected lysate sc-170453 (GenBank: DQ896770.2) Lane 3: mouse Twist1 transfected lysate sc-124377 (GenBank: BC083139.1) Lane 4: human TWIST2 transfected lysate sc-172327 (GenBank: DQ892806.2) Lane 5: human TWIST1 recombinant protein (NCBI: NM_000474.4) Lane 6: human TWIST2 recombinant protein (NCBI: NM_057179.3) -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from ottimo funzionamentoAnticorpo funziona piuttosto bene per WB su lisati di cellule tumorali
Date published: 2022-11-07
Rated 4 out of 5 by from goodUsed for Immunoblot 1:1000 in 5% milk (tbs 0.1%tween) overnight on cell lysates derived from both mesenchymal (20ug total proteins) and epithelial (50ug total proteins) cells, it produces a strong and specific signal.
Date published: 2021-10-04
Rated 5 out of 5 by from Good for WBTested it in murine breast cancer cell lines by western blot. Works well at 1:100 dilution.
Date published: 2021-06-12
Rated 4 out of 5 by from Good for IHC!It worked well for IHC on Paraffin-embedded Human Adrenocortical Carcinoma samples.
Date published: 2019-10-14
Rated 4 out of 5 by from Good antibodyThis antibody is so good, I'm happy to get this result by using this antibody.
Date published: 2019-03-19
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twist 抗体 (Twist2C1a) is rated 4.2 out of 5 by 34.
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