TUSC3 Double Nickase Plasmid (h): sc-405571-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TUSC3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TUSC3 Double Nickase Plasmid (h) and TUSC3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TUSC3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TUSC3 Antibody (D-9): sc-390566
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TUSC3 Double Nickase Plasmid (h) | sc-405571-NIC | 20 µg | $410.00 | |||
TUSC3 Double Nickase Plasmid (h2) | sc-405571-NIC-2 | 20 µg | $410.00 |
TUSC3 (tumor suppressor candidate 3) encodes an endoplasmic reticulum membrane protein that functions as a subunit of the oligosaccharyltransferase (OST) complex, supporting N-linked glycosylation and protein quality control. By influencing glycoprotein folding and ER homeostasis, TUSC3 contributes to proteostasis pathways that intersect with ER stress signaling and cellular differentiation programs. Altered TUSC3 expression or loss-of-function has been associated with dysregulated glycosylation and has been reported in studies of neurodevelopmental phenotypes and cancer biology, making it a relevant target for mechanistic research. In human cells, TUSC3 perturbation is commonly used to probe how ER-associated glycosylation impacts receptor maturation, secretory pathway function, and stress-adaptive responses.
TUSC3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TUSC3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TUSC3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TUSC3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TUSC3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.