Date published: 2025-9-8
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tsg 101 Antibody (51) - Western Blotting - Image 397510
tsg 101 抗体 (51): sc-136111. K-562 (A), Hep G2 (B), MCF7 (C) 和 HEK293 (D) 全细胞裂解液中 tsg 101 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
tsg 101 抗体 (51): sc-136111. K-562 (A), Hep G2 (B), MCF7 (C) 和 HEK293 (D) 全细胞裂解液中 tsg 101 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

tsg 101 抗体 (51): sc-136111

4.1(9)
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说明书
  • tsg 101抗体(51)是小鼠单克隆IgG1 κ, 在35篇文献中引用，规格为200 µg/ml
  • 免疫human物种的tsg 101的氨基酸229-319
  • 推荐用于 mouse, rat, human 和 canine 来源的tsg 101 WB 和 IP检测
  • 看 tsg 101 (C-2): sc-7964，可查看有标记的 tsg 101 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取tsg 101 (51): sc-136111的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是tsg 101 Antibody (51) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与tsg 101 Antibody (51) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

tsg 101抗体（51）是一种IgG1κ小鼠单克隆tsg 101抗体（也称为tsg 101抗体或TSG101抗体），可通过WB和IP检测小鼠、大鼠和人源的tsg 101。tsg 101抗体（51）可以以非偶联抗tsg 101抗体的形式获得。正常细胞转化为恶性细胞可能是由于编码具有关键调节功能的蛋白质的基因突变引起的。例子包括视网膜母细胞瘤基因产物（Rb p110）、p53、VHL和APC。使用一种新的克隆策略，可以分离编码选择性隐性表型的先前未知的基因，从而鉴定出一种额外的肿瘤抑制基因。这种基因被称为肿瘤易感基因101的tsg 101，编码一种stathmin结合结构域蛋白。当使用反义mRNA阻断该生长抑制基因在NIH/3T3细胞中的表达时，细胞呈现转化表型，并在SL6小鼠中具有致瘤性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

tsg 101 抗体 (51) 参考文献:

  1. TSG101/哺乳动物 VPS23 和哺乳动物 VPS28 直接相互作用，并被招募到 VPS4 诱导的内体。  |  Bishop, N. and Woodman, P. 2001. J Biol Chem. 276: 11735-42. PMID: 11134028
  2. 人类 TSG101 不能取代酿酒酵母 VPS23 在质膜蛋白质量控制中的作用  |  Blanco, S. and Lazo, PA. 2003. FEMS Microbiol Lett. 221: 151-4. PMID: 12725919
  3. ESCRT-I亚基TSG101控制病毒RNA的内体-胞浆释放。  |  Luyet, PP., et al. 2008. Traffic. 9: 2279-90. PMID: 18817529
  4. ESCRT和Rim途径都需要Yarrowia lipolytica Vps23的相同结构域，但后者需要Vps23 UEV结构域与Rim8/PalF相互作用。  |  Blanchin-Roland, S. 2011. FEMS Yeast Res. 11: 473-86. PMID: 21539706
  5. 新隐球菌需要ESCRT蛋白Vps23从血红素中获取铁，形成胶囊并产生毒性。  |  Hu, G., et al. 2013. Infect Immun. 81: 292-302. PMID: 23132495
  6. 康乃馨意大利环斑病毒p36复制酶相关蛋白中独特的N端序列与宿主细胞ESCRT-I成分Vps23相互作用。  |  Richardson, LG., et al. 2014. J Virol. 88: 6329-44. PMID: 24672030
  7. 肿瘤易感基因（TSG）-101的螺旋-螺旋结构域的稳定性。  |  White, JT., et al. 2017. Biochemistry. 56: 4646-4655. PMID: 28776372
  8. ESCRT-I亚基Tsg101在经典猪瘟病毒的进入和复制过程中发挥新的双重作用  |  Liu, CC., et al. 2021. J Virol. 95: PMID: 33328308
  9. P1 启动子 IGF-1 多态性与子宫内膜异位症中 IGF-1, IGF-R, LSF 和 TSG 101 的表达谱。  |  Kwasniewski, W., et al. 2022. Ginekol Pol. 93: 775-786. PMID: 35072229
  10. 类真核Vps23的起源塑造了阿斯加德古菌中ESCRT与泛素系统之间古老的功能相互作用  |  Lu, Z., et al. 2024. Cell Rep. 43: 113781. PMID: 38358888

订购信息

产品名称产品编号规格价格数量收藏夹

tsg 101 抗体 (51)

sc-136111
200 µg/ml
$316.00

tsg 101 (51): m-IgGκ BP-HRP 套装

sc-521328
200 µg Ab, 40 µg BP
$354.00

tsg 101 (51): m-IgG1 BP-HRP 套装

sc-533488
200 µg Ab; 20 µg BP
$354.00

What is the dilution range for primary antibody tsg101.?

Asked by: Deepika
Thank you for your question. The dilution range is dependent on the application. Please find the dilution range in the product datasheet for each application, which can be downloaded from this link: https://datasheets.scbt.com/sc-136111.pdf
Answered by: Technical Support
Date published: 2019-01-06

What blocking solution should I use with tsg 101 (51): sc-136111 monoclonal antibody for Western blot?

Asked by: jenniferc
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 1 out of 5 by from Negative result on TE4 cells proteinsI tested this TSG101 antibody on protein extracted from TE-4 cells by western blot. Antibody was diluted 1:200 as suggested in BSA 5% and incubated o/n at 4°C. However, it didn't work and no signal was shown.
Date published: 2021-04-29
Rated 4 out of 5 by from works for western blotI used this antibody on total extracts and exosmose (MCF7 cells). It gives a good band at the expected size, using 1:1000 dilution in TBST-5%milk.
Date published: 2020-02-14
Rated 5 out of 5 by from Worked well for exosomesI have used this antibody for exosomes, I got clear bands
Date published: 2019-07-15
Rated 4 out of 5 by from quite good for IF!I did Immunofluorescence with this antibody: I could see a signal but quite poor at 1/100 dilution and with PFA fixation (so maybe a better signal with 1/50 or 1/20 dilutions) but no signal with MetOH fixation!!!
Date published: 2019-02-13
Rated 4 out of 5 by from Good western resultI received a free sample of tsg101 and used this for exosome verification. Strong clear bands were seen for all 6 cell lines. Will use again.
Date published: 2017-10-12
Rated 5 out of 5 by from Good Western BlotThis antibody produced a clear western blot for human samples in our test
Date published: 2016-12-21
Rated 5 out of 5 by from Perfect resultAntibody of Santa Cruz has very good quality,I believe it can bring me a wonderful experimental result.Thank you.
Date published: 2016-11-04
Rated 4 out of 5 by from Strong band seen in WB using KStrong band seen in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-04-04
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tsg 101 抗体 (51) is rated 4.1 out of 5 by 9.
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