TRP1 Antibody (F-6): sc-166791

TRP1 Antibody (F-6) is a mouse monoclonal IgG1 antibody that detects TRP1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TRP1, or tyrosinase-related protein 1, plays a crucial role in the biosynthesis of melanin, working alongside other proteins such as tyrosinase and TRP2 to facilitate pigment production. Located within the melanosomes of melanocytes, TRP1 is integral to the melanin synthesis pathway, contributing to the stability and activity of the multi-enzyme complex that includes tyrosinase. TRP1′s structure features a transmembrane domain and metal-binding sites, which are essential for enzymatic function. Additionally, TRP1 undergoes post-translational modifications that can influence activity and stability, making TRP1 a key player in pigmentation regulation. Deficiencies in TRP1 can lead to pigmentation disorders, highlighting TRP1′s importance in maintaining normal skin and hair color. Through interactions with other melanin biosynthesis proteins, TRP1 is vital for understanding mechanisms underlying pigmentation and related genetic conditions.

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TRP1 Antibody (F-6) References:

1. Cloning of Trp1beta isoform from rat brain: immunodetection and localization of the endogenous Trp1 protein.  |  Wang, W., et al. 1999. Am J Physiol. 276: C969-79. PMID: 10199829
2. Nonselective colony-color assays for HIS3, LEU2, LYS2, TRP1 and URA3 in ade2 yeast strains using media with limiting nutrients.  |  Myers, TA. and Nickoloff, JA. 1999. Biotechniques. 26: 850-4. PMID: 10337474
3. Trp1-dependent enhancement of salivary gland fluid secretion: role of store-operated calcium entry.  |  Singh, BB., et al. 2001. FASEB J. 15: 1652-4. PMID: 11427516
4. Endogenously expressed Trp1 is involved in store-mediated Ca2+ entry by conformational coupling in human platelets.  |  Rosado, JA., et al. 2002. J Biol Chem. 277: 42157-63. PMID: 12196544
5. Interaction of STIM1 with endogenously expressed human canonical TRP1 upon depletion of intracellular Ca2+ stores.  |  López, JJ., et al. 2006. J Biol Chem. 281: 28254-64. PMID: 16870612
6. Construction of an Arxula adeninivorans host-vector system based on trp1 complementation.  |  Steinborn, G., et al. 2007. J Biotechnol. 127: 392-401. PMID: 16934902
7. TRP1 interacting PDZ-domain protein GIPC forms oligomers and is localized to intracellular vesicles in human melanocytes.  |  Kedlaya, RH., et al. 2006. Arch Biochem Biophys. 454: 160-9. PMID: 16962991
8. SOX-10 and TRP-1 expression in feline ocular and nonocular melanomas.  |  Orlandi, M., et al. 2024. Vet Pathol. 61: 712-720. PMID: 38613415
9. Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia.  |  Bertolotto, C., et al. 1998. Mol Cell Biol. 18: 694-702. PMID: 9447965
10. Molecular interactions within the melanogenic complex: formation of heterodimers of tyrosinase and TRP1 from B16 mouse melanoma.  |  Jiménez-Cervantes, C., et al. 1998. Biochem Biophys Res Commun. 253: 761-7. PMID: 9918801

The preferred TRP1 antibody is TRP1 Antibody (TA99): sc-58438.