tropomodulin 1 CRISPR/Cas9 KO Plasmid (m): sc-423427
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- tropomodulin 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the tropomodulin 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
tropomodulin 1 CRISPR/Cas9 KO Plasmid (m) | sc-423427 | 20 µg | $397.00 |
Overview
Mouse Tmod1 encodes tropomodulin 1, an actin filament pointed-end capping protein that cooperates with tropomyosin to stabilize and define filament length in differentiated cytoskeletal networks. By limiting actin subunit exchange at pointed ends, Tmod1 contributes to myofibril organization, membrane-associated actin architecture, and tension-dependent cellular remodeling. These functions position Tmod1 within pathways governing actin cytoskeleton dynamics, sarcomere assembly, and cell shape control. Altered regulation of actin filament stability is broadly relevant to studies of muscle structure and contractile dysfunction, as well as cytoskeletal contributions to cell motility and mechanobiology.
tropomodulin 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tmod1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tmod1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tmod1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish tropomodulin 1 protein expression.
This CRISPR knockout system enables efficient generation of Tmod1-deficient cell models for investigation of tropomodulin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tmod1 exon(s) critical for tropomodulin 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tmod1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by tropomodulin 1 CRISPR/Cas9 KO Plasmid (m) and tropomodulin 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tmod1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by tropomodulin 1 HDR Plasmid (m) and tropomodulin 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tmod1 homology arms to support homology-directed repair at defined Tmod1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.