Tris Buffered Saline with Tween 20: powder for 500 ml of 10X A convenient prepared buffer for use in various applications.

Tris Buffered Saline with Tween 20: powder for 500 ml of 10X

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同义词: 10X TBST (powder)
应用; A convenient prepared buffer for use in various applications
* 参考分析证明 大量特定数据 (包括水 含量).
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Tris Buffered Saline with Tween 20: powder for 500 ml of 10X is a high-quality powder mix for preparation of 10X buffer concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20: 1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)

Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)


参考文献

用法 :
Reconstitute powder in ddH20 and empty contents of Tween-20 vial (rinse vial thoroughly) into liquid, bringing final volume to 500 ml (10X). Filter solution through a 0.22 μm filter into a sterile flask. Makes 5 L of 1X TBST buffer.

Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. Final (1X) pH 7.4 ±0.2 (may need slight pH adjustment).
物理状态 :
Solid
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
保存 :
Store at room temperature
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
1
MDL 号码 :
MFCD00678198

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